The verification of the applicability of NPHS2/SYNPO ratio for diagnosis of FSGS and MCD
Authors:
M. Šafaříková 1,2; E. Pazourková 2; A. Hořínek 1; J. Reiterová 1,2; D. Maixnerová 2; E. Honsová 3; J. Zadražil 4; J. Štekrová 1; M. Kohoutová 1; V. Tesař 2
Authors‘ workplace:
Ústav biologie a lékařské genetiky, 1. lékařská fakulta UK v Praze a Všeobecná fakultní nemocnice v Praze
1; Klinika Nefrologie, 1. lékařská fakulta UK v Praze a Všeobecná fakultní nemocnice v Praze
2; Institut klinické a experimentální medicíny v Praze
3; III. Interní klinika, Lékařská fakulta Univerzity Palackého v Olomouci a Fakultní nemocnice v Olomouci
4
Published in:
Klin. Biochem. Metab., 24, 2016, No. 2, p. 63-66
Overview
Objective:
The aim of this study was the verification of the applicability of NPHS2/SYNPO ratio for diagnosis of focal segmental glomerulosclerosis (FSGS) and minimal change disease (MCD) in Czech patients and identification of new markers for distinguishing between MCD and FSGS.
Material and methods:
Our study was performed on 24 samples from patients with FSGS and MCD. Gene expressions were determined using relative quantitative real-time PCR and TaqMan® Array Micro Fluidic Cards and normalized to the reference gene GAPDH.
Results and conclusion:
Our results did not find any significant difference in the podocin/synaptopodin ratio and in any other studied genes between FSGS and MCD.
Keywords:
FSGS, MCD, gene expressions, quantitative real-time PCR.
Sources
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3. Korbet, S. M., Schwartz, M. M., Lewis, E. J. Primary Focal Segmental Glomerulosclerosis: Clinical Course and Response to Therapy, Am. J. Kidney Dis., 1994, 23, 6, p. 773-783.
4. D’Agati, V. D., Fogo, A. B., Bruijn, J. A., Jennette J. C. Pathologic classification of focal segmental glomerulosclerosis: a working proposal. Am. J. Kidney Dis., 2004, 43, 2, p. 368-382.
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9. Schmid, H., Henger, A., Cohen, C. D. Gene expression profiles of podocyte-associated molecules as dia-gnostic markers in acquiered proteinuric diseases. J. Am. Soc. Nephrol., 2003, 14, 11, p. 2985-2966.
10. Bennett, M. R., Czech, K. A., Arend, L. J., Witte, D. P., Devarajan, P., Potter, S. S. Laser capture microdissetion-microarray analysis of focal segmental glomerulosclerosis glomeruli. Nephron Exp. Nephrol., 2007, 107, 1, p. e30-e40.
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Clinical Biochemistry and Metabolism
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