VZTAH HLADIN SOLUBILNÍCH CYTOADHEZIVNÍCHMOLEKUL sVCAM-1 A sICAM-1 V SÉRU K VYBRANÝMPŮSOBKŮM CYTOKINOVÉ SÍTĚ U MNOHOČETNÉHOMYELOMU

Relation of Soluble Cytoadhesive Molecules sVCAM-1 and sICAM-1in Serum to Selected Parameters of the Cytokine Network at Multiple Myeloma

Background.
Contemporary, not very frequent studies have brought only few and often inconsistent findings aboutthe significance of cytoadhesive molecules (CAM) type „vascular cell adhesion molecule-1” (VCAM-1) and„intercellular cell adhesion molecule-1” (ICAM-1) at multiple myeloma (MM). The aim of the study was to interpretrelations among levels of soluble forms of VCAM-1 and ICAM-1 in serum of the peripheral blood (SPB) and inserum of the aspirated bone marrow (SABM) to concentrations of selected elements of the cytokine network (IL-2,sIL-2R, IL-6, sIL-6R, and TNF-a) during different phases of MM disease and to recognise whether there are somespecific relations of these factor in both tissue fluids.Methods and Results. Two groups of patients with MM were analysed: the group of 64 patients examined indifferent phases of MM disease and group of 39 patients examined when the disease was diagnosed (age medianwas 63 and 64 years, male to female relation was 1.6 and 1.3 to 1.0). CAM, cytokine and their soluble receptor levelswere estimated using ELISA method. Increased levels of sVCAM-1 in APB were found in 87.5 and 87 % of patients;in both groups sVCAM-1 medians almost twice exceeded the upper limits of normality (1180 and 1295 ng/ml).Levels in SABM were always higher (1347 and 1546 ng/ml) than that in SPB. Higher values of sICAM-1 in SPBhad 35 and 33 % of patients; sICAM-1 medians in SPB and in SABM did not exceed in either group the upper limitsof normality (691 ng/ml) and they did not differ significantly (519 vs 476 and 518 vs 500 ng/ml). Statistical analysis(Pearson’s correlation quotient, p

Key words:
multiple myeloma, soluble cytoadhesive molecules, sVCAM-1, sICAM-1, serum cytokines.