Cryopreservation of blood – history, methods and the current status
Authors:
M. Bohoněk
Authors‘ workplace:
Oddělení hematologie, biochemie a krevní transfuze, Ústřední vojenská nemocnice – Vojenská fakultní nemocnice Praha
Published in:
Transfuze Hematol. dnes,19, 2013, No. 1, p. 44-50.
Category:
Comprehensive Reports, Original Papers, Case Reports
Overview
This article presents a brief overview of methods used for the cryopreservation of blood, their development over the years and their application. Cryopreservation of blood is presented as a method, which solves various problems in the blood transfusion service, as storage of frozen blood can significantly extend its shelf life, provided that special conditions are guaranteed during the freezing process in order to prevent possible cell damage. The main application of such cryopreservation is in military medicine and blood crisis policy, but it also plays a role in special transfusiology fields, such as the storage of rare red blood cell types and the long-term storage of selected autologous blood. Thanks to modern technological procedures, manipulation and logistics of cryopreserved products following thawing have been simplified in recent years, thus improving access to such products.
Key words:
cryopreservation of blood, cryopreservation of red cells, cryopreservation of platelets, cryoprotective agents
Sources
1. Amer KA, Pepper AS, Prowse CHV. Effect of Different Resuspension Media on the Post-thaw Characteristics of Frozen Blood. British J of Haem, 1980, 44: 635-644.
2. Smith AU. Prevention of haemolysis during freezing and thawing of red blood cells. Lance 1950; 11: 1990-911.
3. Pouchet MFA. Recherches expérimentales sur la congélation des animaux, Journ. L´Anat et Physiol 1866; iii: 1-36.
4. Huggins CE. Frozen Blood. Europ Surg Res 1969; 1: 3-12.
5. Loevelock JE. The Haemolysis of human red blood cells by freezing and thawing. Biochem biophys Acta 1953; 10: 414.
6. Loevelock JE. The Protective Action of Neutral Solutes aginst Haemolysis by Freezing and Thawing. Biochem J 1954; 56: 265-270.
7. Levin RL, Cravalho FG, Huggins CE. Effect of hydratation on the water content of human erythrocytes. Biophys J 1976; 16: 1411-14260.
8. Hess JR, Hill HR, Oliver CK, Lippert LE, Greenwalt TJ. The effect of two additive solutions on the postthaw storage of RBCs. Transfusion 2001 Jul; 41(7): 923-7.
9. Sputtek A. Kryokonservierung von Blutzellen. Transfusionsmedizin, 2nd. ed. (Ed.: C.Mueller-Eckhardt), 125-167, Springer, Berlin 1996.
10. Polge F, Smith AU, Parkers AJ, Revival of spermatozoa after vitrification and dehydratation at low temperatures, Nature, 1949, 164:666-669.
11. Valeri CR. Frozen Blood. New Engl J Med 1966; 25: 425-431.
12. Tullis JL, Ketchei MM, Pyle HM, Penneli GB, Gibson JG, Tinch RJ. Studies on the in vivo survival of glycerolized and frozen human red blood cells, J of the Am Med Association 1958; 168: 399-404.
13. Valeri CR, Pivacek LE, Cassidy GP, Rango G. The survival, function, and hemolysis of human RBCs stored at 4°C in additive solution (AS-1, AS-3, or AS-5) for 42 days and then biochemically modified, frozen, thawed, washed, and stored at 4°C in sodium chloride and glucose solution for 24 hours. Transfusion 2000; 40(11): 1341-5.
14. Valeri CR, Ragno G, Pivacek LE, et al. A multicenter study of in vitro and in vivo values in human RBCs frozen with 40-percent (wt/vol) glycerol and stored after deglycerolization for 15 days at 4 degrees C in AS-3: assessment of RBC processing in the ACP 215. Transfusion 2001; 41(7): 933-9.
15. Lelkens, CCM, Koning JG, de Kort B, Floot IBG, Noorman F. Experiences with frozen blood products in the Netherlands military. Transfus Apher Sci, 2006; 34: 289-98.
16. Bohoněk M, Petráš M, Turek I, et al. Parametry kvality kryokonzervovaných erytrocytů rekonstituovaných v roztoku AS-3 (Nutricel). Trans Hemat dnes 2006; 12: 208-216.
17. Bohoněk M, Petráš M, Turek I, et al. Vliv typu odběru krve a deleukotizace na kvalitu kryokonzervovaných erytrocytů. Trans Hemat dnes 2007; 13: 200-208.
18. Bohoněk M, Petráš M, Turek I, et al. Quality evaluation of frozen apheresis red blood cell storage with 21-day postthaw storage in additive solution 3 and saline-adenine-glucose-mannitol: biochemical and chromium-51 recovery measures. Transfusion 2010; 50: 1007-1013.
19. Mollison PL, Sloviter HA. Successful transfusion of previously frozen human red cells. Lancet 1951; 2: 862.
20. Tullis JL, Haynes L, Pyle H, et al. Clinical use of frozen blood. Arch Surg 1960; 81: 169.
21. Pert JH, Schork PK, Moore R. A new method of low temperature blood preservation using liquid nitrogen and glycerol sucrose additive. Clinical Research 1963; 11: 197.
22. Lionetti FJ, Hunt SM. Cryopreservation of Human Red Cells in Liquid Nitrogen with Hydroxyethyl Starch. Cryobiology 1975; 12: 110-118.
23. Greenwood CT, Muir DD, Whitcher HW. Hydroxyethyl Starch as a Cryoprotective Agent for Human Red Blood Cells. The Relation Between the Molecular Properties and the Cryoprotective Effect. Starch-Stärke 1977; 29: 343-347.
24. Sputtek A, Singbartl G, Langer R, Schleinzer W, Henrich HA, Künl P. Cryopreservation of red blood cells with the non-penetrating cryoprotectant hydroxyethylstarch. Cryo Letters 1995; 16: 283-288.
25. Horn EP, Sputtek A, Standl T, Rudolf B, Kühnl P. Schulte Transfusion of autologous, hydroxyethyl starch-cryopreserved red blood cells. Anesthesia & Analgesia 1997; 85: 739-745.
26. Guide to the preparation, use and quality assurance of blood components, Council of Europe, Recommendation No.R(95) 15
27. Commission Directive 2004/33/EC of March 2004, Annex IV.
28. Hank TH, Palk IK. The Cryopresevation and Thawing of Red Blood Cells Using 25% Hydroxyethyl. Korean J Lab Med 2004; 24: 314-319.
29. Meryman HT, Hornblower M. Red Cell Recovery and leukocyte Depletion Following Washing of Frozen-Thawed Red Cells. Transfusion 1973; 13: 388-393.
30. Lecak J, Scott K, Young C, Hannon J, Acker JP. Evaluation of red blood cells stored at -80 degrees C in excess of 10 years. Transfusion 2004; 44: 1306-13.
31. Handin RI, Valeri CR. Improved Viability of Previously Frozen Platelets. Blood 1972; 40: 509-513.
32. Daly PA, Schiffer CA, Aisner J, Wierni PH. Successful transfusion of platelets cryopreserved for more than 3 years. Blood 1979; 54: 1023-1027.
33. Taylor MA. Cryopreservation of platelets: an in-vitro comparison of four methods. J Clin Pathol 1981; 34: 71-75.
34. Owens M, Werner E, Holme S, Afferbach C. Membrane glycoproteins in cryopreserved platelets. Vox Sang 1994; 67: 28-31.
35. Bohoněk M. Možnosti kryokonzervace - doporučené postupy u potřeby autologních odběrů a dovezených přípravků. Trans Hemat dnes 2007; 13: 174.
Labels
Haematology Internal medicine Clinical oncologyArticle was published in
Transfusion and Haematology Today
2013 Issue 1
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