RNA vaccine – clinical studies
Authors:
S. Dudová 1,2; J. Michálek 1,2,3; R. Hájek 1,2,4
Authors‘ workplace:
Laboratoř experimentální hematologie a buněčné imunoterapie, Oddělení klinické hematologie, Fakultní nemocnice Brno, 2Lékařská fakulta Masarykovy Univerzity Brno, 3Fakultní nemocnice Brno, 4Interní hematoonkologická klinika
Fakultní nemocnice Brno
1
Published in:
Transfuze Hematol. dnes,14, 2008, No. 1, p. 28-33.
Category:
Comprehensive Reports, Original Papers, Case Reports
Overview
Immunotherapy using dendritic cells develops is considered as one of the possible treatments of patients with tumour diseases in the last years. These antigen presenting cells play important role in the induction of primary immune reaction on specific antigens. Data from in vitro studies of the efficiency of vaccines of dendritic cells loaded with individual tumour RNA provides promising information and the base for further dendritic cell vaccines development. Impact of RNA-transfected cell vaccine on T-cell immunity and preparation of therapeutic anticancer responses was first studied on murine models and acquired results subsequent clinical studies on humans. Following survey summarizes studies focused on the use of RNA vaccines in tumour patients.
Key words:
dendritic cell, RNA vaccine, transfection
Sources
1. Dudová S, Michálek J, Hájek R. RNA nádorové vakcíny na bázi dendritických buněk. Klinická onkologie 2005; 5: 178-83.
2. Nair SK, Heiser A, Boczkowski D, et al. Induction of cytotoxic T cell responses and tumor immunity against unrelated tumors using telomerase reverse transcriptase RNA transfected dendritic cells. Nat Med 2000; 6(9): 1011–7.
3. Heiser A, Maurice MA, Yancey DR, et al. Human dendritic cells transfected with renal tumor RNA stimulate polyclonal T-cell responses against antigens expressed by primary and metastatic tumors. Cancer Res 2001; 61(8): 3388-93.
4. Gilboa, E. The risk of autoimmunity associated with tumor immunotherapy. Nat. Immunol. 2001; 2:789–792.
5. Javorovic M, Pohla H, Frankenberger B, et al. RNA transfer by electroporation into mature dendritic cells leading to reactivation of effector-memory cytotoxic T lymphocytes: a quantitative analysis. Mol Ther 2005; 12(4): 734-43.
6. Grunebach F, Muller MR, Nencioni A, et al. Delivery of tumor-derived RNA for the induction of cytotoxic T-lymphocytes. Gene Ther 2003; 10(5): 367-74.
7. Li X, Zhao X, Fang Y, et al. Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 1998; 273(52): 34970-5.
8. Boczkowski D, Nair SK, Snyder D, et al. Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo. J Exp Med 1996; 184(2): 465-72.
9. Heiser A, Coleman D, Dannull J, et al. Autologous dendritic cells transfected with prostate-specific antigen RNA stimulate CTL responses against metastatic prostate tumors. J Clin Invest 2002; 109(3): 409-17.
10. Asai T, Storkus WJ, Whiteside TL, et al. Evaluation of the modified ELISPOT assay for gamma interferon production in cancer patients receiving antitumor vaccines. Clin Diagn Lab Immunol 2000; 7: 145–154.
11. Nair SK, Morse M, Boczkowski D, et al. Induction of tumor-specific cytotoxic T lymphocytes in cancer patients by autologous tumor RNA-transfected dendritic cells. Ann Surg 2002; 235: 540–549.
12. Morse MA, Nair SK, Mosca PJ, et al. Immunotherapy with autologous, human dendritic cells transfected with carcinoembryonic antigen mRNA. Cancer Invest 2003; 21(3): 341-9.
13. Glaspy JA. Therapeutic options in the management of renal cell carcinoma. Semin Oncol 2002; 29: 41-46.
14. Su Z, Dannull J, Heiser A, et al. Immunological and clinical responses in metastatic renal cancer patients vaccinated with tumor RNA-transfected dendritic cells. Cancer Res 2003; 63(9): 2127-33.
15. Su Z, Dannull J, Yang BK, et al. Telomerase mRNA-Transfected Dendritic Cells Stimulate Antigen-Specific CD8+ and CD4+ T Cell Responses in Patients with Metastatic Prostate Cancer 2005, 174: 3798-3807.
16. Wong P, Pamer EG. CD8 T cell responses to infectious pathogens. Annu Rev Immunol 2003; 21: 29-70.
17. Mu LJ, Kyte JA, Kvalheim G, et al. Immunotherapy with allotumour mRNA-transfected dendritic cells in androgen-resistant prostate cancer patients. Br J Cancer 2005; 93(7): 749-56.
18. Kyte JA, Mu L, Aamdal S, et al. Phase I/II trial of melanoma therapy with dendritic cells transfected with autologous tumor-mRNA. Cancer Gene Ther 2006; 13(10): 905-18.
19. Caruso DA, Orme LM, Neale AM, et al. Results of a phase 1 study utilizing monocyte-derived dendritic cells pulsed with tumor RNA in children and young adults with brain cancer. Neuro-oncol 2004; 6(3): 236-46.
20. Caruso DA, Orme LM, Amor GM, et al. Results of a Phase I study utilizing monocyte-derived dendritic cells pulsed with tumor RNA in children with Stage 4 neuroblastoma. Cancer 2005; 103(6): 1280-91.
21. Geiger C, Regn S, Weinzierl A, et al. A generic RNA-pulsed dendritic cell vaccine strategy for renal cell carcinoma. J Transl Med 2005; 3: 29.
Labels
Haematology Internal medicine Clinical oncologyArticle was published in
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