Preparation of Extractof Peripheral Blood Progenitor Cells to the Autologous Transplantation in Patients withSolid Tumours – Our Experience

Overview

High - dose chemotherapy with autologous transplantation of peripheral blood progenitor cells isa possible treatment for the high-risk oncology patients. Peripheral blood stem cells (PBSC) have beenseparated on Autotransfusion detachment in IKEM Prague since November 1996. By the end ofSeptember 2002, 64 patients (47 man and 17 women) with solid tumours were indicated for collections.PBSC separations were commenced at value CD 34+ peripheral blood cells over 20 x 103/ml aftermobilization by combination of chemotherapy with growth factors - G-CSF (Neupogen in dose 15µg/kg/day). There were 247 separations provided on the whole on the Haemonetics MCS 3p and MCS+separators, respectively. Both types of separators were compared. All the patients were given extractPBSC extracts back: 40 patients after the application of high – dose chemotherapy and 24 patients afterthe application of only intensificated therapy. This procedure appears to be very perspective for thisgroup of patients in the future.

Key words:
peripheral blood progenitor cells, high - dose chemotherapy, autologous transplantations