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Preparation and Use of Tumor Antigen from Pathological Immunoglobulinfor Patients with Multiple Myeloma


Authors: R. Musilová 1;  E. Táborská 2;  L. Bourková 1;  T. Büchler 1,3;  L. Hanák 1;  L. Kovářová 1,3;  H. Novotná 4;  M. Penka 1;  R. Hájek 1,3
Authors‘ workplace: Laboratoř experimentální hematologie a buněčné imunoterapie, Oddělení klinické hematologie, FN Brno, 2Lékařská fakulta MU, Katedra lékařské chemie a biochemie, 3Interní hematoonkologická klinika, FN Brno, 4Oddělení klinické biochemie, FN Brno 1
Published in: Transfuze Hematol. dnes,, 2002, No. 3, p. 0.
Category:

Overview

Idiotype vaccination can be used as immunotherapy for B-cell malignancies, including multiplemyeloma. Malignant plasma cells produce monoclonal immunoglobulin that can be detected in theserum and/or urine and is tumour-specific. To isolate the myeloma Id protein we used affinity chromatographyon a protein G column. Id was eluted with 0.2M glycine-HCl. The protein was more than 90 %pure as determined by SDS-polyacrylamide gel electrophoresis. Id protein was conjugated with KLHin the presence of 0.1% glutaraldehyde. The conjugation product was administered in the preclinicalexperiment to test vaccination as a part of the clinical protocol under preparation.

Key words:
monoclonal immunoglobulin, idiotypic protein, affinity chromatography

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Labels
Haematology Internal medicine Clinical oncology
Topics Journals
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