New diagnostic tools in cervical cancer screening (ThinPrep Pap test)

Czech version

Authors: Iva Kinkorová Luňáčková
Authors‘ workplace: Bioptická laboratoř s. r. o., Plzeň
Published in: Prakt Gyn 2013; 17(2): 157-160
Category: Oncogynecology: Review Article

Overview

Carcinoma of uterine cervix belongs to the frequent women malignities, due to its localization as the well accessible area and the effort is to identify women who are at increased risk of cervical cancer precursors. Routinely used is the smear from uterine cervix, when the cells after staining are observed in the optical microscope and based on especially nuclear characteristics are evaluated by the Bethesda 2001 system. Besides conventional cytology (C) we can now in Czech Republic use the methods of liquid based cytology (LBC), which allows us to take more examinations from one sample (e.g. HPV testing). The purpose of this article is to acquaint the gynecologists with the LBC techniques, focusing on ThinPrep Pap test and with describing the principle of computer image analysis while evaluating the gynecological cytology.

Key words:
carcinoma of uterine cervix – HPV – Imager – liquid based cytology – Pap test – screening carcinoma of uterine cervix – ThinPrep Pap Test – uterine cervix

Sources

1. Ondruš J, Dvořák V. Screening karcinomu děložního hrdla – teoretické předpoklady. Čes Gynek 2010; 75(1): 62–64.

2. Dvořák V, Ondruš J. Screening děložního hrdla – současný stav. Čes Gynek 2010; 75(1): 65–68.

3. Mac Demay R. The Pap Test. American Society for Clinical Pathology: Singapore 2005. ISBN 0–89189–420–9.

4. Samama B, Schaeffer C, Boehm N. p16(INK4A) expression in relation to human papillomavirus in liquid-based cervical smears. Gynecol Oncol 2008; 109(2): 285–290.

5. Roelens J, Reuschenbach M, von Knebel Doeberitz M. p16(INK4A) immunocytochemistry versus human papillomavirus testing for triage of women with minor cytologic abnormalities. Cancer Cytopathol 2012; 120(5): 294–307.

6. Benevolo M, Vocaturo A, Mottolese M et al. Clinical role of p16INK4a expression in liquid-based cervical cytology:correlation with HPV testing and histologic diagnosis. Am J Clin Pathol 2008; 129(4): 606–612.

7. Samarawardana P, Dehn D, Singh M et al. p16(INK4a) is superior to high-risk human papillomavirus testing in cervical cytology for the prediction of underlying high-grade dysplasia. Cancer Cytopathol 2010; 118(3): 146–156.

8. Miller FS, Nagel LE, Kenny-Moynihan MB. Implementation of the ThinPrep Imaging System in a high volume metropolitan laboratory. Diag Cytopath 2007; 35(4): 213–217.

9. Lozano R. Comparison of computer-assisted and manual screening of cervical cytology. Gynecol Oncol 2007; 104(1): 134–138.

10. Dziura B, Quinn S, Richard K. Performance of an imaging system vs. manual screening in detection of squamous intraepithelial lesions of the uterine cervix. Acta Cytol 2006; 50(3): 309–311.