Cryopreservation of cells and tissues in assisted reproduction
Authors:
J. Žáková; P. Ventruba; E. Lousová; I. Crha; R. Hudeček
Authors‘ workplace:
Gynekologicko-porodnická klinika, LF MU a FN Brno
Published in:
Prakt Gyn 2011; 15(1): 16-21
Category:
Review Article
Overview
The paper reviews a past development and current options available for cryopreservation of germinal cells, embryos and tissues in assisted reproduction. Indications for cryopreservation and storage of sperm, oocytes, embryos and testicular and ovarian tissue are presented. Two cooling and thawing methods are described (slow computer-assisted freezing, vitrification), including the use of cryoprotectants (glycerol, DMSO, PROH). Prior to storage in a cryostorage vessel, serological examination of HIV1,2; hepatitis and syphilis to prevent cross contamination of biological material has to be performed.
Key words:
cryopreservation – vitrification – cryoprotectant – thawing – sperm – oocyte – embryo – ovarian tissue
Sources
¨1. Polge C, Smith AU, Parkes AS. Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature 1949; 164(4172): 666.
2. Lovelock JE, Polge C. The immobilization of spermatozoa by freezing and thawing and the protective action of glycerol. Biochem J 1954; 58(4): 618–622.
3. Whittingham DG, Leibo SP, Mazur P. Survival of mouse embryos frozen to -196 °C and -289 °C. Science 1972; 178: 411–414.
4. Trounson A, Mohr L. Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature 1983; 305(5936): 707–709.
5. Zeilmaker GH, Alberda A, Van Gent I. Two pregnancies following transfer of intact frozen-thawed embryos. Fertil Steril 1984; 42(2): 293–296.
6. Produckt Catalog COOK Medical. Asisted reproductive technology 2009: 1–72.
7. ORIGIO Product Catalog, Medicult media, Humagen Pipets and MidAtlantic Devices. Version 1. March 1. 2010. Available from: http://viewer.zmags.com/publication/9945a62a#/9945a62a/1.
8. Žáková J, Ventruba P, Crha I et al. Sperm Donation Programme at the Centre of Assisted Reproduction in Brno: 1995–2005 results. Scripta Medica Brno 2005; 6: 323–328.
9. Crha I, Ventruba P, Žáková J et al. Kryokonzervace spermatu před gonadotoxickou léčbou - 11 let zkušeností. Česk Gynek 2007; 72(5): 320–326.
10. Crha I, Ventruba P, Žáková J et al. Survival and infertility treatment in male cancer patients after sperm banking. Fertil Steril 2009; 91 (6): 2344–2348.
11. Crha I, Kraliková M, Melounová J et al. Seminal plasma homocysteine, folate and cobalamin in men with obstructive and non-obstructive azoospermia. J Assist Reprod Genet 2010; 27(9–10): 533–538.
12. Donnelly ET, McClure N, Lewis SE. Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity. Fertil Steril 2001; 76(5): 892–900.
13. O’Connell MO, McClure N, Lewis SE. The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Hum Reprod 2001; 17(3): 704–709.
14. Chen C. Pregnancy after human oocyte cryopreservation. Lancet 1986; 1(8486): 884–886.
15. Tucker MJ. Human oocyte and embryo cryopreservation. The Past and Science of Assisted Reproductive Techniques. London, New York: Taylor § Francis 2004.
16. Huser M, Crha I, Hudeček R et al. Ovarian tissue cryopreservation – new opportunity to preserve fertility in female cancer patients. Eur J Gynaecol Oncol 2007; 28(4): 249–255.
17. Huser M, Crha I, Ventruba P et al. Prevention of ovarian function damage by a GnRH analogues during chemotherapy in Hodgkin lymphoma patients. Hum Reprod 2008; 23(4): 863–868.
18. Parmegiani L, Garello C, Granella F et al. Long-term cryostorage does not adversely affect the outcome of oocyte thawing cycles. Reprod Biomed Online 2009; 19(3): 374–379.
19. Revel A, Safran A, Laufer N et al. Twin delivery following 12 years of human embryo cryopreservation: case report. Hum Reprod 2004; 19(2): 328–329.
20. Feldschuh J, Brassel J, Durso N et al. Successful sperm storage for 28 years. Fertil Steril 2005; 84(4): 1017.
Labels
Paediatric gynaecology Gynaecology and obstetrics Reproduction medicineArticle was published in
Practical Gynecology
2011 Issue 1
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