Histological and Histochemical Analysis of Retraction Pocket Pars Tensa of Tympanic Membrane in Children
Authors:
M. Urík 1,2; P. Hurník 3,4,5; D. Žiak 3,5; J. Macháč 1,2; I. Šlapák 1,2; O. Motyka 6; O. Vaculová 3
Authors‘ workplace:
Klinika dětské otorinolaryngologie, Lékařská fakulta, Masarykova univerzita, Brno
1; Fakultní nemocnice Brno
2; Ústav patologie, Fakultní nemocnice Ostrava
3; Ústav patologie, Lékařská fakulta, Ostravská univerzita, Ostrava
4; CGB laboratoř, a. s., Ostrava-Vítkovice
5; Centrum nanotechnologie, VŠB, Technická univerzita Ostrava
6
Published in:
Otorinolaryngol Foniatr, 66, 2017, No. 1, pp. 35-39.
Category:
Original Article
Overview
Aims:
Histological and histochemical analysis of retraction pocket of pars tensa of tympanic membrane in children. Identification of morphological abnormalities in comparison with a healthy tympanic membrane was investigated as well as identification of signs typical for cholesteatoma and support for a retraction theory of cholesteatoma formation.
Study design:
A prospective study analyzing 31 samples of retraction pockets taken during surgery. Departments: University Hospital, Children’s Medical Centre.
Methods:
Samples of retraction pockets were processed by a standard process for light microscopy, stained by hematoxilin-eosin. Van Gieson’s stain was used for differential staining of collagen, Verhoeff’s stain for elastic fiber tissues, Alcian blue for acidic polysaccharides and PAS (Periodic Acid Schiff) method for basement membrane polysaccharides.
Results:
The following findings were observed in the samples of retraction pockets: 1 hyperkeratosis (100 %), 2 hypervascularisations (100 %), 3 subepithelial fragmented elastic fibres (96 %), 4 myxoid changes (87 %), 5 subepithelial inflammatory infiltration (84 %) 6 rete pegs (71 %), 7 papillomatosis (71 %) 8 intraepithelial inflammatory cellularizations (48 %), 9 intraepithelial spongiosis (16 %) and 10 parakeratosis (3 %). No basement membrane continuity interruptions were observed. Length and thickness of retraction pocket, thickness of epidermis, occurrence of rete pegs and frequency of fragmented elastic fibres was higher in a grade III than grade II (according to Charachon).
Conclusion:
Morphological abnormalities in the structure of retraction pockets in comparison with a healthy tympanic membrane were described. The changes are typical for a structure of cholesteatoma, supporting retraction theory of its origin. Our observations show that it is inflammation that plays a key role in the pathogenesis of retraction pocket. The frequency of some of the changes increases with the stage of retraction pocket (II-III according to Charachon). Basement membrane continuity interruptions are not typical for retraction pockets.
Keywords:
retraction pockets, histological analysis, cholesteatoma, children
Sources
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Labels
Audiology Paediatric ENT ENT (Otorhinolaryngology)Article was published in
Otorhinolaryngology and Phoniatrics
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