Expression of Bcl-2 (B-Cell Lymphoma 2) Gene in Primary Pleomorphic Adenoma in Relation to Its Histogenesis
Authors:
K. Obtulovičová 1; I. Stárek 2; A. Skálová 3; A. Kališ 4; M. Sičák 1; P. Čmilanský 5
Authors‘ workplace:
Klinika otorinolaryngológie a chirurgie hlavy a krku, Ústredná vojenská nemocnica a fakultná nemocnica, Ružomberok
1; ORL klinika LF UP a FN v Olomouci
2; Šiklův patologický ústav LF UK, Plzeň
3; Ústav patologickej anatómie, Ústredná vojenská nemocnica a fakultná nemocnica, Ružomberok
4; Poliklinika Sekčov, s. r. o., ORL a SOFYC clinic, Prešov
5
Published in:
Otorinolaryngol Foniatr, 63, 2014, No. 3, pp. 176-181.
Category:
Original Article
Overview
Pleomorphic adenoma is morphologically characterized as biphasic tumour, including epithelial and mesenchymal components. At present its epithelial origin is accepted. Pluripotent unicellular theory is the most plausible tumorigenesis theory. According this theory, the steam cell is the origin for renewal of mature salivary gland tissue as well as for tumour growth. This cell is the basis for epithelial as well as mesenchymal tumour components. Bcl-2 (b-cell lymphoma 2) gene is the steam cell marker that protects cell against apoptosis. In retrospective study, the authors followed up immunoexpression and distribution of bcl-2 oncoprotein in single cell population of 24 primary pleomorphic adenomas. Bcl-2 positivity was detected in 95.8% pleomorphic adenomas (91.7% in epithelial and 62.5% in mesenchymal components). The most intensive immunostaning was found in the outer cells of well differentiated excretory duct resembling formations. The cells of the inner layer were always negative. Week to moderate reaction was present in cells at periphery of excretory-like ductal formations as well as in “intermediate” cells in some unstructured solid regions. The majority of mesenchymal cells were negative, minority showed week to moderate positivity, only occasionally with strong immunoreaction. The occasional foci of squamous cell metaplasia were bcl-2 negative. The study confirmed the pluripotent unicellular theory. Authors consider bcl-2 gene as the key element in pleomorphic adenoma genesis, in cooperation with other antiapoptotic mechanisms.
Keywords:
bcl-2 gene, pleomorphic adenoma, histogenesis, apoptosis
Sources
1. Al-Rawi, N. H., Omer, H., Al Kawas, S.: Immunohistochemical analysis of P53 and bcl-2 in benign and malignant salivary gland tumors. Oral. Pathol. Med., 36, 2010, s. 48-55.
2. Aoki, T., Tsukinoki, K., Karakida, K., Ota, Y., Otsuru, M., Kaneko, A.: Expression of cyclooxygensae-2, Bcl-2 and Ki-67 in pleomorphic adenoma with special reference to tumor proliferation and apoptosis. Oral. Oncol., 40, 2004, s. 954-959.
3. Bell, K., Van Arsdale, C., Bronner, M., Pasha, T., Furth, E.: The relationship of the expression of PCNA and bcl-2 in inflammatory and neoplastic lesions of the gastrointestinal tract. Modern. Path., 7, 1994, 1, s. 57A.
4. Bronner, M., Brentnall, T., Burner, G., Rabinovich, P.,Rubin, C., Hagitt, R., Hockenberry, D.: Bcl-2 protooncogen in ulcerative colitis associated neoplastic progression: correlation with morphology, p53, and DNA content. Lab. Invest., 57, 1994, 2, s. 78-91.
5. Carlinfante, G., Lazzaretti, M., Ferrari, S., Bianchi, B., Crafa, P.: p53, bcl-2 and Ki-67 expression in adenoid cystic carcinoma of the palate. A clinoco-pathologic study of 21 cases with longterm follow-up. Pathol. Res. Pract., 200, 2004, 11/12, s. 791-799.
6. Chatla, Ch., Jhala, N. C., Katkoori, V. R. et al.: Recurrence and survival predictive value of phenotypic expression of Bcl-2 varies with tumor stage of colorectal adenocarcinoma. Am. J. Pathol., 1, 1994, s. 241-250.
7. Cruz Perez, D. E., Pires, F. R., Alves, F. A., Almeida, O. P., Kowalski, L. P.: Salivary gland tumors in children and adolescents: a clinicopathologic and immunohistochemical study of fiftythree cases. Int. J. Ped. Otorhinolaryngol., 68, 2004, s. 895-902.
8. Dardick, I., Van Nostrand, A.W., Phillips, M. J.: Histogenesis of salivary gland pleomorphic adenoma with an evaluation of the role of the myoepithelial cell. Hum. Pathol., 13, 1982, s. 62-75.
9. Debiec-Rychter, M., Van Valckenborgh, I., Van denBroeck, C. H. et al.: Histologic localization of PLAG1 (pleomorphic adenoma Gene 1) in pleomorphic adenoma of the salivary gland: cytogenetic evidence of common origin of phenotypically diverse cells. Lab. Invest., 81, 2001, s. 1289-1297.
10. Farnebo, L., Jedlinski, A., Ansell, A. et al.: Proteins and single nucleotide polymorphisms involved in apoptosis, growth control and DNA repair predict cisplatin sensitivity in head nad neck cancer cell lines. Int. J. Mol. Med., 42, 2009, s. 549-556.
11. Gordon-Nunez, M. A., Godoy, G. P., Soares, R. C.: Immunohistochemical expression of PCNA, p53 and bcl-2 in pleomorphic adenomas. Int. J. Morphol., 26, 2008, s. 567-572.
12. Hockenbery, D. M.: Bcl-2, a novel regulator of cell death. Bioessays., 17, 1995, s. 631-638.
13. Joensuu, H., Pylkkänen, L., Toikkanen, S.: Bcl-2 protein expression and long-term survival in breast cancer. Am. J. Pathol., 145 1994, 5, s. 1191-1198.
14. Kas, K., Voz, M. L., Roijer, E. et al.: Promoter swapping between the genes for a novel zinc protein and beta-catenin in pleomorphic adenomas with t(3;8)(p21;q12) translocations. Nat. Genet., 15, 1997, 5, s. 170-174.
15. Keshgegian, A. A., Johnston, E., Cnaan, A.: Bcl-2 oncoprotein positivity and high MIB-1 (Ki-67) proliferative rate are independent predictive markers in prostate carcinoma. Am. J. Clin. Pathol., 110, 1998, s. 443-449.
16. Kolář, Z.: Molekulární patologie nádorú. Epava Olomouc, 2003.
17. Nagao, T., Sugano, I., Ishida, Y. et al.: Basal cell adenoma of the salivary glands: comparison with basal cell adenoma through assessment of cell proliferation, apoptosis, and expresssion of p53 and bcl-2. Cancer., 23, 1998, s. 439-447.
18. Obtulovičová, K., Stárek, I.: Význam bcl-2 génu pri nádoroch hlavy a krku a nádoroch slinných žliaz. Otorinolaryngol. Chir. Hlavy. Krku., 5 2011, 1, s. 24-27.
19. Pammer, J., Horvat, R., Weninger, W., Ulrich, W.: Expression of bcl-2 salivary glands and salivary gland adenomas. A contribution to the reserve cell theory. Path. Res. Pract., 191, 1995, 3, s. 35-41.
20. Pezizella, F., Turley, H., Kuzu, I. et al.: Bcl-2 protein in non small cell lung carcinoma. N. Engl. J. Med., 1993, 329, s. 690-694.
21. Seifert, G., Brocheriou, C., Cardesa, A., Evenson, J. W.: WHO international histological classification of tumours. Tentative histological classification of salivary gland tumours. Pathol. Res. Pract. 1990, 186, s. 555-581.
22. Soini, Y., Tormanen, U., Paakko, P.: Apoptosis is inversely related to bcl-2 but not to bax expression in salivary gland tumors. Histopathol., 32, 1998, 1, s. 28-34.
23. Stárek, I., Černý, L., Simpson, R. W. H et al.: Choroby slinných žláz (1. vydanie). Grada Publishing, spol. s r.o., Praha, 2000.
24. Sunardhi-Widyaputra, S., Van Damme, B.: Immuno-histochemical pattern of bcl-2 and PTHrP-positive cells in primary, in recurrent and in carcinoma in pleomorphic adenomas. Path. Res. Pract., 191, 1995, s. 1186-1191.
25. Tovey, S. M., Dunne, B., Witton, C. J., Cooke, T. G., Bar-lett, J. M. S.: HER4 in breast cancer: comparison of antibodies against intra- and extracelullar domains in HER4. Breast. Cancer. Research., 19, 2006, 8, s. 527-532.
26. Wenig, M. B.: Atlas of head and neck pathology (2nd edn). Saunders Elsevier Philadelphia, 2008.
27. Yanez, M., Roa, I., Garcia, M., Ibacache, G., Villaseca, M.: Bcl-2 protein expression in salivary gland tumors. Rev. Med. Chil., 127, 1999, 2, s. 139-142.
28. Yin, H. F., Okada, N., Tagaki, M.: Apoptosis and apoptotic-relataed factors in mucoepidermoid carcinoma of the oral minor salivary glands. Pathol. Int., 50, 2000, s. 603-609.
29. Zbären, P., Stauffer, E.: Pleomorphic adenoma of the parotid gland: Histopathologic analysis of the capsular characteristics of 218 tumors. Head. Neck., 29, 2007, s. 751-757.
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Otorhinolaryngology and Phoniatrics
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