Influence of 0.5 mT/50Hz Magnetic Field on Inhibition of Adherence of Leukocytes Obtained from Blood of Patients with Head and Neck Cancer

Overview

Peripheral blood of 76 patients with head and neck cancer was sampled before the beginning of therapy during 2002 and 2003. CD 4 lymphocytes were separated from the blood. Their ability to adhere to the surface in relation to the presence of antigen (Leukocyte Adherence Inhibition Assay - LAI) was observed. A specific antigen made from the relevant tumor tissue (mitochondrial antigen of tumor cells) was used as well as a non-specific antigen made from serum of inbred mice of C3H/H2K strain, infected with LDH virus (capsid antigen). The results were evaluated by means of non-adherence index (NAI). The experiment was repeated after exposure of the substrate to magnetic sinusoid field (MSP) supply voltage frequency of 0,5 mT induction. The results were evaluated in the same way and compared by t-test for two independent samples. At the same time, identical experiments were performed with CD4 lymphocytes of a control group, obtained from sera of healthy individuals (a group of 76 voluntary blood donors). Results of the measurement showed that CD4 lymphocytes, obtained from the blood of patients with larynx and pharynx cancer display, after exposure to MSP of supply voltage frequency of 0.5mT induction, significantly higher ability to adhere to the surface. The same effect was observed in the control group of CD4 lymphocytes, obtained from blood donors. However, the NAI values in the latter group were significantly lower without exposure as well as after the exposure to MSP. Assuming that the adhering ability of lymphocytes is a manifestation of cell-mediated immunity (CMI), it may be concluded that MSP positively influences CMI in the case of MSP supply voltage frequency of 0.5mT.

Key words:
larynx and pharynx cancer, magnetic sinusoid field – Leukocyte Adherence Inhibition assay