The most frequent methods used for DNA methylation analysis
Authors:
Marcela Chmelařová; Vladimír Palička
Authors‘ workplace:
Univerzita Karlova v Praze, Lékařská fakulta a FN Hradec Králové, Ústav klinické biochemie a diagnostiky
Published in:
Čas. Lék. čes. 2011; 150: 442-445
Category:
Review Articles
Overview
There is an important relationship between the degree of DNA methylation and gene activity in cancer. Number of techniques of DNA methylation analysis has been reported. The aim of this study was to review methodologies that can be used to monitoring DNA methylation changes. We reviewed techniques important for monitoring of DNA methylation changes of CpG islands in gene promoter regions. Advantages, disadvantages and potential use of these techniques are described.
Key words:
DNA hypermethylation, bisulfite conversion, MSP, MS-MLPA.
Sources
1. Mareš J, Sedláček Z, Goetz P. Metylace DNA a nádorová onemocnění. Čas Lék čes 2000; 139(10): 291–294.
2. Esteller M. Epigenetics in Cancer. N Engl J Med 2008; 358(11): 1148–1159.
3. Bird A. DNA methylation patterns and epigenetic memory. Genes Dev 2002; 16(1): 6–21.
4. Herman JG, Graff JR, Myöhänen S, Nelkin BD, Baylin SB. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA 1996; 93(18): 9821–9826.
5. Dejeux E, El Abdalaoui H, Gut IG, Tost J. Identification and quantification of differentially methylated loci by the pyrosequencing technology. Methods Mol Biol 2009; 507: 89–205.
6. Wojdacz TK, Dobrovic A. Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high--throughput assessment of methylation. Nucleic Acids Res 2007; 35(6): e41.
7. Waalwijk C, Flavell RA. MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites. Nucleic Acids Res 1978; 5(9): 3231–3236.
8. von Kanel T, Gerber D, Schaller A, Baumer A, Wey E, Jackson CB, Gisler FM, Heinimann K, Gallati S. Quantitative 1-step DNA methylation analysis with native genomic DNA as template. Clin Chem 2010; 56(7): 1098–1106.
9. Nygren AO, Ameziane N, Duarte HM, Vijzelaar RN, Waisfisz Q, Hess CJ, Schouten JP, Errami A. Methylation-specific MLPA (MS-MLPA): simultaneous detection of CpG methylation and copy number changes of up to 40 sequences. Nucleic Acids Res 2005; 33(14): e128.
10. Thu KL, Pikor LA, Kennett JY, Alvarez CE, Lam WL. Methylation analysis by DNA immunoprecipitation. J Cell Physiol 2010; 222(3): 522–531.
Labels
Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management Dental HygienistArticle was published in
Journal of Czech Physicians
Most read in this issue
- Pelvic ring injuries: current concepts of management
- Clinical importance of the IgG4 related disease
- Reactive oxygen and nitrogen species in the clinical medicine
- The most frequent methods used for DNA methylation analysis