#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Principle and Importance of Using the Array CGH in Hematooncology


Authors: M. Jarošová;  H. Pospíšilová;  R. Plachý;  M. Divoká;  M. Holzerová;  T. Papajík;  J. Koptíková 1;  K. Indrák
Authors‘ workplace: Hemato-onkologická klinika LF a FN, Olomouc ;  Centrum biostatistiky a analýz LF a PřF MU, Brno 1
Published in: Čas. Lék. čes. 2006; 145: 9-13
Category: Review Article

Overview

Identification of chromosomal changes and variation in DNA copy number allows us to understand pathogenesis of tumors. To the frequently diagnosed chromosomal changes belong acquired gains and losses of chromosomal regions carring genes involved in cellular proliferation and differentiation as well as oncogenes and tumor suppressor genes. The determination of gene changes is limited by techniques used for their identification. The introduction of genom-wide microarray technology, resolution has rapidly increased. Array comparative genomic hybridization (arrayCGH) offers higher resolution for genome-wide detection of chromosomal alteration and it is able to analyze hundreds to thousands of genes presented on microarray in one experiment. The aim of this study was to perform arrayCGH technology and to stress its value for the identification of chromosomal imbalances in hematological malignancies. 

Key words:
DNA, in situ hybridization, CGH, arrayCGH, microarray, oncogene, tumor suppressor gene.


Labels
Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management Dental Hygienist

Article was published in

Journal of Czech Physicians


Most read in this issue
Topics Journals
Login
Forgotten password

Enter the email address that you registered with. We will send you instructions on how to set a new password.

Login

Don‘t have an account?  Create new account

#ADS_BOTTOM_SCRIPTS#