Serum Phospholipid Fatty Acid Composition and Insulin Action in Type 2 Diabetic Patients

Overview

Background.
A direct relation has been found between the insulin sensitivity and fatty acid (FA) composition of serum and tissue lipids in adult humans. The aim of our study was to confirm the above relation in different groups of type 2 diabetic patients (DM2).Methods and Results. The FA composition of serum phospholipids was measured by gas liquid chromatography at the insulin action during the two-step hyperinsulinemic isoglycemic clamp (1 and 10 mU/kg.min) in a group of 21 netyly diagnosed DM2 subjects (DMN) and in 24 healthy controls (HC). The DMN group consisted of a subgroup of patients treated with hypoglycemic agents (DMH; n=21) and a group treated with the diet only (DMD; n=11). In diabetics, the metabolic clearance rates of glucose at either insulin level (MCRgwsubmax and MCRg,~max) were signi%cantly reduced in comparison with HS (MCRg,~submax - DMN: 5.35±2.7 ml.kg'.miri'; DMH: 5.38±2.17 ml.kg'.miri'; DMD: 5.48±2.35 ml.kg-'.min'; HS: 10.9±3.3 ml.kg'.min:'; ~

Key words:
type 2 diabetes mellitus, insulin resistance, fatty acids, linoleic acid, arachidonic acid