Separation of Haematopoietic Progenitor Cells from Peripheral Blood(PBPC) in Patients with Haematooncological Malignities

Overview

Background.
Transplantations of haematopoietic progenitor cells from peripheral blood (PBPC) are able to ensurehaematopoietic and immunological reconstitution as well as stable long term engraftment. Autologous PBPC areadministered after previous myeloablative chemotherapy to patients with haematological and non-haematologicalmalignancies.The objective of the submitted study was to follow-up the results of autologous separations of PBPC in patientswith a good effect of mobilisation therapy. The authors evaluated in PBPC concentrates the content of cell parametersneeded for transplantation. In the subsequent part of the trial they mention the times of engraftment after autologoustransplantation.Methods and Results. The authors evaluated parameters of 26 separations of PBPC in 11 haematooncologicalpatients with a good effect of mobilisation therapy and with concentration of CD 34+ cells higher than 20 . 103/mlof peripheral blood. The separations of PBPC were implemented on the Cobe Spectra and Baxter CS 3000 Plusequipment under a standard regime with processing of 12 l blood, i.e. 2.7 total blood volumes of the patients. In thementioned group of patients already from one separation an adequate amount of CD 34+ cells for their transplantationwas obtained. Transplantation doses were prepared on average from two separations and amounted as regardsMNC/kg, CD 34+ cells/kg and CFU-GM/kg to 4.3 . 108, 17.1 . 106and 2.5 . 104. The assembled parametrs correspondto, or in some parameters exceed, recommended amounts for their transplantation.Conclusions. In well mobilised patients under the regime of standard separations adequate amounts of progenitorcells for their autologous transplantation were obtained. Transplantation doses were prepared from two collections.Investigation of pre-separation concentration of CD 34+ cells in the peripheral blood is a reliable indicator for startingPBPC separation. Early post-transplantation results indicate the time of engraftment 10 days on average and minorneed of substitution therapy with blood products.

Key words:
haematopoietic progenitor cells in peripheral blood (PBPC), mobilisation, separation, autologoustransplantation.