Changes in metallothionein levels in patients with malignant prostate cancer – a pilot study

Czech version

Authors: L. Melich ¹; J. Raja ¹; L. Vysloužilová ²; O. Štěpánková ²; J. Čepová ¹; M. Fořtová ¹; K. Kotaška ¹; E. Klapková ¹; J. Werle ¹; R. Průša ¹; R. Kizek ¹
Authors‘ workplace: 2. lékařská fakulta Univerzity Karlovy a Fakultní nemocnice v Motole, Praha Ústav lékařské chemie a klinické biochemie, Přednosta: prof. MUDr. Richard Průša, CSc. ¹; České vysoké učení technické v Praze, Český institut informatiky, robotiky a kybernetiky Oddělení biomedicínského inženýrství a asistivní technologie, Vedoucí oddělení: prof. RNDr. Olga Štěpánková, CSc. ²
Published in: Prakt. Lék. 2023; 103(6): 299-303
Category: Of different specialties

Overview

Aim: To analyse concentrations of metallothionein (MT) in urine samples of patients diagnosed with prostate cancer (CaP).
Methods: Samples were electrochemically analysed by difference pulse voltametry (DPV) method in Brdička’s buffer. Acquired data were then evaluated as an area under the curve (AUC). Concentrations of MT were determinedby calibration curve method.
Results: Metallothioneins are proteins with molecular weight about 10 kDa. Theirbiological significance lies in maintaining homeostasis of metal ions. Their analytical determination is complicated. One of the most effective determination methods is by electrochemistry. Previously mentioned methods were used tostudy the changes of MT concentrations of patients with CaP, and with benign prostate hyperplazia (BHP). Our pilotexperiment determined concentrations of MT in a) healthy controls (n = 13) was 2.9 ± 1.2 µg/mmol of creatinine, b) BHP was 4.7 ± 1.7 μg/mmol of creatinine, c) CaP group (n = 9) was 6.7 ± 1.5 µg/mmolof creatinine. Difference between healthy controls and CaP group was statistically significant (p = 0.0099). Differencebetween BHP and CaP group was also determined (p = 0.0928). Difference between BHP and healthy controls is notvery statistically significant (p = 0.7869).
Conclusion: We were able to demonstrate differences between healthycontrols, BHP patients and CaP patients.Concentrations of MT in CaP patients were elevated, but they need to be studied in more detail.

Keywords:

oxidative stress – thiol compounds – metallomics – electrochemical methods

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adresa pro korespondenci:
prof. Ing. René Kizek, Ph.D., DrSc., MBA
Ústav lékařské chemie aklinické biochemie
2. LF UK a FN Motol
V Úvalu 84, 150 06 Praha 5
e-mail: kizekrene@gmail.com

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Article was published in

General Practitioner

2023 Issue 6