Point-of-care method for measuring infliximab and adalimumab trough levels in inflammatory bowel disease patients on biologic therapy

Czech version

Authors: Kaštylová K. ¹; Černá K. ¹; Luxová Š. ²; Šebek P. ³; Lukáš M. ¹
Authors‘ workplace: Klinické a výzkumné centrum pro střevní záněty, Klinické centrum ISCARE a. s. a 1. LF UK v Praze ¹; GENNET s. r. o., Praha ²; Tani-Gen s. r. o., Praha ³
Published in: Gastroent Hepatol 2022; 76(1): 35-41
Category:
doi: https://doi.org/10.48095/ccgh202235

Overview

Aim of the study: To verify whether the results of infliximab and adalimumab concentration measurements by the point-of-care method ProciseDx correspond with the concentrations determined by enzyme-linked immunosorbent assay (ELISA). Methods: Twenty-three infliximab (IFX) and twenty-two adalimumab (ADL) concentrations were compared. IFX and ADL trough levels were measured by the ProciseDx point-of-care (POCT) system based on the Förster resonance energy transfer (FRET) principle, and by standard immunoassay (ELISA) in a clinical laboratory. Results: Spearman‘s correlation coefficient for IFX R = 0.9400 (P = 0.0001) and the weighted Cohen‘s kappa of 0.93 indicate a good correlation between data measured by two different measurement systems. The same excellent correlation of values was achieved for ADL, where R = 0.9098 (P = 0.0001) and the weighted Cohen‘s kappa is 0.834. Conclusion: The ProciseDx POCT system is a highly reliable method for measuring serum trough IFX and ADL levels in IBD patients. It is easy to operate and fast, it brings more effectiveness to the IBD biological treatment and reduces financial and time burden of this therapy.

Keywords:

infliximab – inflammatory bowel disease – adalimumab – biologic therapy – proactive therapeutic drug monitoring – reactive therapeutic drug monitoring – point-of-care monitoring

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