Assessmentof Phagocytic Leucocyte Activity on a Flow Cytometer by Means of MicrosphericHydrophilic Fluorochrome Labelled Particles

Czech version

Authors: K. Malíčková ¹; Z. Sedláková ²; M. Řeřichová ³; H. Marečková ¹; T. Fučíková ¹
Authors‘ workplace: Ústav klinické imunologie a alergologie, Všeobecná fakultní nemocnice a 1. lékařská fakultaUniverzity Karlovy, Praha2 Ústav makromolekulární chemie, Akademie věd České republiky, Praha 3 ARTIM, spol. s r. o., Praha ¹
Published in: Epidemiol. Mikrobiol. Imunol. , 1999, č. 3, s. 106-110
Category:

Overview

The phagocytic activity was determined in human polymorphonuclear leucocytes using the flowcytometry technique with microspheric hydrophilic particles labelled with fluorescein isothiocya-nate. Non-specific adherence of particles is discussed and normal values of phagocytic activity arepresented. The advantages of this method include simplified sample preparation using whole blood,rapidity of the test, precise phagocytosis quantification and possibility of archiving all findings ina computer.

Key words:
polymorphonuclear leucocytes – phagocytosis – ingestion – flow cytometry.

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Article was published in

Epidemiology, Microbiology, Immunology

1999 Issue 3