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Proof and Detection of Abused Opiates in Urine and Blood of Fatal Cases thin Layer Chromatography with Densitometry


Authors: J. Valuch;  M. Bauer *
Authors‘ workplace: Ústav súdneho lekárstva LF UK a FN, Bratislava, prednosta doc. MUDr. M. Mego, CSc. * Laboratórium súdnej chémie a toxikológie LF UK, Bratislava, vedúci doc. RNDr. M. Bauer, CSc.
Published in: Čes.-slov. Patol., , 1998, No. 4, p. 58-64
Category:

Overview

Urine and blood concentrations of free and total morphin or 6-monoacetylmorphin were presen- ted in fatal cases of morphin type opiates abuse. A solid phase extraction method was developed for isolation of drugs and their metabolities from biological material which used Separcol small columns with non-polar contents SI C 18T. Thin layer chromatography with densitometry anabled screening for quality evaluations. Resultes were compared with those obtained by fluoropolari- zing immunodetection on Abbotts TDxFLx device. Posibility and cause of false positive results were discussed when using initial, screening, commercialy available immunotests.

Key words:
drug abuse - drug screening - morphin - 6-monoacetylmorphin - solid phase SI C 18T extraction - thin layer chromatography - densitometry - immunometods

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Labels
Anatomical pathology Forensic medical examiner Toxicology
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