Molecular Epidemiology of Dermatophytoses in the Czech Republic – Two-Year-Study Results
Authors:
V. Hubka 1,2; T. Větrovský 3; S. Dobiášová 4; M. Skořepová 5; P. Lysková 6; K. Mencl 7; N. Mallátová 8; H. Janouškovcová 9; J. Hanzlíčková 9; R. Dobiáš 4; A. Čmoková 1; J. Stará 5; P. Hamal 10; L. Svobodová 10; M. Kolařík 1,2
Authors‘ workplace:
Katedra botaniky, Přírodovědecká fakulta, Univerzita Karlova v Praze
vedoucí katedry doc. RNDr. Yvonne Němcová, Ph. D.
1; Laboratoř genetiky a metabolismu hub, Mikrobiologický ústav, Akademie věd České republiky, v. v. i., Praha
vedoucí laboratoře Mgr. Miroslav Kolařík, Ph. D.
2; Laboratoř environmentální mikrobiologie, Mikrobiologický ústav, Akademie věd České republiky, v. v. i., Praha
vedoucí laboratoře: RNDr. Petr Baldrian, PhD.
3; Oddělení bakteriologie a mykologie, Centrum klinických laboratoří, Zdravotní ústav se sídlem v Ostravě
vedoucí oddělení RNDr. Vladislav Holec
4; Centrum pro dermatomykózy, Dermatovenerologická klinika 1. lékařské fakulty Univerzity Karlovy
a Všeobecné fakultní nemocnice v Praze, přednosta kliniky prof. MUDr. Jiří Štork, CSc.
5; Laboratoř lékařské mykologie, oddělení parazitologie, mykologie a mykobakteriologie Praha, Zdravotní ústav
se sídlem v Ústí nad Labem, Praha, vedoucí oddělení RNDr. Zuzana Hůzová
6; Oddělení klinické mikrobiologie, Pardubická krajská nemocnice, a. s., Pardubice
primář oddělení MUDr. et Mgr. Eva Zálabská, Ph. D.
7; Pracoviště parazitologie a mykologie, Centrální laboratoře Nemocnice České Budějovice, a. s.
ředitel MUDr. Břetislav Shon
8; Ústav mikrobiologie, Fakultní nemocnice Plzeň-Lochotín
přednosta ústavu RNDr. Karel Fajfrlík Ph. D.
9; Ústav mikrobiologie, Lékařská fakulta Univerzity Palackého v Olomouci
přednosta ústavu prof. MUDr. Milan Kolář, Ph. D.
10
Published in:
Čes-slov Derm, 89, 2014, No. 4, p. 167-174
Category:
Clinical and laboratory Research
Overview
The aim of the study was to evaluate the spectrum of causative agents of the main clinical forms of dermatophytoses in the Czech Republic by molecular genetic methods (MGM). During two years (from July 2011 to June 2013), 3255 cultivation specimens were positive for dermatophytes. The highest number of specimens was isolated from tinea unguinum (55,5%), then tinea corporis (29,2%), tinea pedis (14,6%) and tinea capitis (0,7%). The identification of isolated species (n = 672) except for Trichophytom rubrum (n = 2563) was performed by MGM (PCR fingerprinting or sequencing of ITS segments of rDNA). For T. rubrum only morphologically non-clear isolates were identified (n = 189). In total, 14 species of dermatophytes were identified. The most important change noticed compared to previous results was an increased detection of Arthroderma benhamiae in the cases of tinea corporis (22,9%) and tinea capitis (29,2%). Geophilic species caused only 1,3% of all infections even if this group comprised the highest number of species with two newly described ones and also Microsporum persicolor and M. fulvum wich are usually overlooked in the morphological examination.
Key words:
Arthroderma benhamiae – DNA sequencing – geophilic dermatopytes – ITS region of rDNA – PCR-fingerprinting – tinea capitis – tinea corporis – tinea pedis – tinea unguinum – zoophilic dermatophytes
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Labels
Dermatology & STDs Paediatric dermatology & STDsArticle was published in
Czech-Slovak Dermatology
2014 Issue 4
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