Photodynamic Antiseptic Effect of Photoactive Nanofibers in the Treatment of Leg Ulcers
Authors:
P. Arenberger 1; M. Arenbergerová 1; S. Gkalpakiotis 1; J. Hugo 1; M. Bednář 2; P. Kubát 3; J. Mosinger 4,5
Authors‘ workplace:
Dermatovenerologická klinika 3. LFUK a FNKV, Praha
přednosta prof. MUDr. Petr Arenberger, DrSc., MBA
1; Ústav lékařské mikrobiologie 3. LFUK a FNKV, Praha
přednosta doc. MUDr. Marek Bednář, CSc.
2; Ústav fyzikální chemie J. Heyrovského AV ČR, Praha
ředitel prof. RNDr. Zdeněk Samec, DrSc.
3; Katedra anorganické chemie, Přírodovědecká fakulta UK, Praha
děkan fakulty prof. RNDr. Bohuslav Gaš, CSc.
4; Ústav anorganické chemie AV ČR, v. v. i., Řež
ředitelka Ing. Jana Bludská, CSc.
5
Published in:
Čes-slov Derm, 87, 2012, No. 5, p. 176-182
Category:
Pharmacologyand Therapy, Clinical Trials
Overview
Nanofiber textiles doped with 5,10,15,20-tetraphenylporphyrin (TPP) photosensitizer prepared by electrospinning from highly hydrophilic polyurethane TecophilicTM were characterized with microscopic methods and time-resolved spectroscopy. Complete inhibition of bacterial growth of the three tested bacterial strains on the surface of nanofiber textiles with encapsulated TPP was observed on illuminated samples in vitro. The antibacterial effect is based on photogeneration of cytotoxic singlet oxygen O2(1Δg) upon illumination by visible light. The antibacterial activity was also clinically tested in 162 patients with chronic leg ulcers. The application of illuminated doped nanofiber textiles yielded to a 35% decrease in wound size as assessed with computer aided wound tracing. The wound related pain estimated by the visual analogue scale declined by 71%. This methodology seems to be an effective alternative to topical antibiotics and antiseptics in chronic wounds and shows broad spectrum of application in medicine, particularly in dermatology, burn units and surgery.
Key words:
leg ulcer – nanofabrics – photosensitizer – photodynamic therapy
Sources
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Dermatology & STDs Paediatric dermatology & STDsArticle was published in
Czech-Slovak Dermatology
2012 Issue 5
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