In vitro Diagnostics of Allergic Contact Dermatitis – Examination of Peripheral Blood Mononuclear Cells of Nickel Allergic Patients by the Means of Lymphocyte Transformation Test and ELISA for Determination of Interleukin 5

Czech version

Authors: D. Buchvald ¹; L. Lundeberg ²
Authors‘ workplace: Detská dermatovenerologická klinika LF UK a DFNsP, Bratislava prednosta doc. MUDr. T. Danilla, CSc. ¹; Unit of Dermatology and Venereology, Department of Medicine, Karolinska University Hospital, Stockholm, Švédsko prednosta prof. Mona Stahle-Backdahl, MD, PhD. ²
Published in: Čes-slov Derm, 83, 2008, No. 1, p. 16-24
Category: Clinical and laboratory Research

Overview

The diagnostics of the etiology of allergic contact dermatitis (ACD) is currently almost solely based on the results of patch tests, which, however, have several disadvantages. Therefore there is a real need for an alternative diagnostic method. The aim of our study was to characterize the potential utility of two different laboratory methods for in vitro diagnosis of contact nickel hypersensitivity. Peripheral blood mononuclear cells of patients with nickel allergic contact dermatitis and a control group of healthy persons without contact allergy were examined and the characteristics of lymphocyte transformation test and interleukin 5 (IL-5) ELISA test were studied as potential diagnostic methods. Our results suggest that both tests might be suitable for in vitro diagnostics of ACD caused by nickel. However, it is necessary to increase the number of patients enrolled into study to confirm this conclusion.

Key words:
diagnostics – allergic contact dermatitis – lymphocyte transformation test – ELISA – IL-5

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Article was published in

Czech-Slovak Dermatology

2008 Issue 1