Local sperm antibodies and general levels of immunoglobulins in ovulatory cervical mucus

Czech version

Authors: P. Hušáková ¹; Z. Ulčová-Gallová ²; K. Bibková ²; Z. Mičanová ²
Authors‘ workplace: Nemocnice Teplice, o. z., oddělení gynekologicko-porodnické, přednosta MUDr. M. Šoltés ¹; Gynekologicko-porodnická klinika LF UK a FN, Plzeň, přednosta doc. MUDr. Z. Rokyta, CSc. ²
Published in: Ceska Gynekol 2010; 75(4): 334-337

Overview

Objective:
The aim of our preliminary study was to compare the levels of total local sIgA and IgG with activity of detected sperm antibodies in ovulatory cervical mucus (OCM).

Setting:
Department of Gyneacology and Obstetrics, Medical School and Faculty Hospital, Charles University, Plzen.

Methods:
We screened samples of OCM from 12 pacients aged 26–31 (29,6 years on average) by radial immunodifusion (RID) to determine s IgA and IgG. Indirect MAR test was used for detection of spermagglutinationg antibodies.

Results:
We found out by RID the average concentration of sIgA in OCM 567,84 mg/l (0 -1250,47) and the average concentration of IgG in OCM 23,57 mg/l (8,74–47,99). Antibody activity against sperm cells dominates in IgA with 6 pacients, in IgA with 1 patient, in IgA and IgG together with 1 infertile woman and in IgA and IgM isotypes together with 1 patient. No local sperm antibodies were determined with 3 patients.

Conclusion:
We proved the hypothesis, that the levels of spermagglutinating antibodies do not correlate with findings of total sIgA and IgG in OCM with our patients.

Key words:
sperm agglutinating antibodies, ovulatory cervical mucus, i-MAR-test.

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