Expression of p53, Ki-67, bcl-2, c-erb-2, estrogen, and progesterone receptors in endometrial cancer

Czech version

Authors: R. Pilka; I. Míčková ¹; M. Lubušký ²; M. Dušková ¹; M. Říčánková ³; Milan Kudela ^{1 1}
Authors‘ workplace: Porodnicko gynekologická klinika, LF UP a UP, Olomouc ¹; Ústav lékařské genetiky, LF UP a FN Olomouc ²; Ústav patologické anatomie, LF UP a FN Olomouc ³
Published in: Ceska Gynekol 2008; 73(4): 222-227

Overview

Objective:
To assess the immunohistochemical expression of p53, bcl-2, c-erb-2, Ki-67, estrogen and progesterone receptors in endometrial cancer patients.

Design:
Experimental study.

Setting:
Department of Obstetrics and Gynecology, Institute of Human Genetics, Department of Pathology, Palacky University Medical School and University Hospital, Olomouc.

Methods:
We studied 103 cases of primary untreated endometrial carcinoma in which the p53, bcl-2, c-erb-2, Ki-67, estrogen and progesterone receptor antigens were investigated by an immunohistochemical method. We evaluated the correlations among the immunohistochemical staining assessed by histoscore, and the age, grading, depth of invasion, stage of the neoplasia and extrauterine disease.

Results:
Mean age was 67 years (range 35-90). p53, bcl-2, c-erb-2, Ki-67, estrogen and progesterone receptors were positive in 49 (48%), 81 (79%). 18 (17%), 99 (96%), 73 (70%) and 87 (84%) patients respectively. There was no clear association between immunohistochemical parameters and the age of patients. p53 and Ki-67 overexpression was found to be related to poor grade of differentiation, deeper myometrial invasion, advanced stage of neoplasia and extrauterine spread of disease. Immunostaining for bcl-2 correlated inversely with FIGO stage, while c-erb-2 was overexpressed in tumors with deeper myometrial invasion. Estrogen and progesterone receptor positive tumors showed a statistically significant association with clinicopathological parameters of better clinical outcome.

Conclusion:
The overexpression of p53 and Ki-67 seems to indicate more malignant phenotype, while bcl‑2 and c-erb-2 may have a limited role in the identification of high-risk tumors.

Key words:
endometrial cancer, immunohistochemistry, prognostic factors.

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