#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Testing of the potentially probiotic lactobacilli for use in food supplements


Authors: Andrea Bilková;  František Bilka;  Hana Kiňová Sepová;  Andrea Balážová
Published in: Čes. slov. Farm., 2013; 62, 40-45
Category: Original Articles

Overview

Some lactobacilli strains are used as probiotics in food industry and in dietary supplements. Eight lactobacilli strains, originated from the stomach of the lamb and goatling, are tested for their potential use in human and/or veterinary medicine in our department. Sanguinarine is a major alkaloid of Papaveraceae suspension cultures. For its antimicrobial, antifungal, anti-plaque and anti-inflammatory properties sanguinarine found application in dental preparations. A mixture of sanguinarine with other alkaloids is used for appetite stimulation of livestock. This paper is focused on testing of selected lactobacilli sensitivity to sanguinarine, isolated from the opium poppy suspension cultures. L. reuteri KO5 demonstrated the lowest sensitivity (MIC 0.6 mg.l-1). Other less sensitive strains were L. murinus C, L. mucosae D and L. plantarum KG4 (MIC 0.3 mg.l-1). For a combination of more strains of microorganisms in one preparation it is necessary to know their ability to influence their growth and survival. To test the mutual influence of lactobacilli, the streak line method on agar plates was used. The results show that the strains L. reuteri E, L. plantarum KG1 a L. reuteri KO4m are the most suitable ones for the use in combinations.

Keywords:
Lactobacillus spp. • probiotics • food supplements • sanguinarine


Sources

1. Coudeyras S., Jugie G., Vermerie M., Forestier Ch. Adhesion of human probiotic Lactobacillus rhamnosus to cervical and vaginal cells and interaction with vaginosis-associated pathogens. Infect. Dis. Obstet. Gynecol. 2008; http://www.hindawi.com/journals/idog/ 2008/549640/ (13.11.2012)

2. Myllyluoma E., Ahonen A. M., Korpela R., Vapaatalo H., Kankuri E. Effects of multispecies probiotic combination on Helicobacter pylori infection in vitro. Clin. Vaccine Immunol. 2008; 15, 1472–1482.

3. Lin P. P., Hsieh Y. M., Tsai Ch. Ch. Antagonistic activity of Lactobacillus acidophilus RY2 isolated from healthy infancy feces on the growth and adhesion characteristics of enteroaggregative Escherichia coli. Anaerobe 2009; 15, 122–126.

4. Sepp E., Stšepetova J., Smidt I., Rätsep M., Kõljalg S., Lõivukene K., Mändar R., Jaanimäe L., Löhr H.I., NatČs O.B., Naaber P. Intestinal lactoflora in Estonian and Norwegian patients with antibiotic associated diarrhea. Anaerobe 2011; 17, 407–409.

5. Bilková A., Kiňová Sepová H., Bilka F., Balažová A. Bakteriocíny produkované baktériami mliečneho kvasenia. Čes. a slov. Farm. 2011; 60, 65–72.

6. Kiňová Sepová H., Dubničková M., Bilková A., Bukovský M., Bezáková L. Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb. Braz. J. Microbiol. 2011; 42, 1188–1196.

7. Nikawa H., Makihira S., Fukushima H., Nishimura H., Ozaki Y., Ishida K., Darmawan S., Hamada T., Hara K., Matsumoto A., Takemoto T., Aimi R. Lactobacillus reuteri in bovine milk fermented decreases the oral carriage of mutans streptococci. Int. J. Food Microbiol. 2004; 95, 219–223.

8. Caglar E., Kavaloglu S. C., Kuscu O. O., Sandalli N., Holgerson P. L., Twetman S. Effect of chewing gums containing xylitol or probiotic bacteria on salivary mutans streptococci and lactobacilli. Clin. Oral Investig. 2007; 11, 425–429.

9. Ouwehand A. C., Vesterlund S. Antimicrobial components from lactic acid bacteria. In: Salminen S., von Wright A., Ouwehand A. C. eds. Lactic Acid Bacteria: Microbiological and functional aspects, 3rd ed. New York: Marcel Dekker, Inc. 2004.

10. Kiňová Sepová H., Bilková A., Bilka F., Bezáková L. Antimikróbne pôsobiace látky produkované baktériami mliečneho kvasenia. Čes. a slov. Farm. 2010; 59, 155–159.

11. Bilková A., Bilka F., Bezáková L. Enzymológia tvorby benzylizochinolínových alkaloidov. Čes. slov. Farm. 2005; 54, 17–22.

12. Dostál J., Slavík J. Novější poznatky o sanguinarinu a příbuzných alkaloidech. Chem. Listy 2000; 94, 15–20.

13. Zdařilová A., Malíková J., Dvořák Z., Ulrichová J., Šimánek V. Kvartérní isochinolinové alkaloidy sanguinarin a chelerythrin. Účinky in vitro a in vivo. Chem. Listy 2006; 100, 30–41.

14. Simeon S., Rios J. L., Villar A. Pharmacological activities of benzophenanthridine and phenanthrene alkaloids. Pharmazie 1989; 44, 593–597.

15. Walterová D., Ulrichová J., Valka I., Vičar J., Vavrečková C., Táborská E., Harkrader R. J., Meyer D. L., Černá H., Šimánek V. Benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine: Biological activities and dental care applications. Acta Univ. Palacky Olomouc Fac. Med. 1995; 139, 7–16.

16. Newton S. M., Lau C., Gurcha S. S., Besra G. S., Wright C. W. The evaluation of fourty-three plant species for in vitro antimicrobial activities. J. Ethnopharmacol. 2002; 79, 57–67.

17. Mahady G. B., Pendland S. L., Stoia A., Chadwick L. R. In vitro susceptibility of Helicobacter pylori to isoquinoline alkaloids from Sanguinaria canadensis and Hydrastis canadensis. Phytother. Res. 2003; 17, 217–221.

18. Merschjohann K., Sporer F., Steverding D., Wink M. In vitro effect of alkaloids on bloodstream forms of Trypanosoma brucei and T. congolense. Planta Med.2001; 67, 623–627.

19. Navarro V., Delgado G. Two antimicrobial alkaloids from Bocconia arborea. J. Ethnopharmacol. 1999; 66, 223–226.

20. Bilková A., Kiňová Sepová H., Bukovský M., Bezáková L. Antibacterial potential of lactobacilli isolated from a lamb. Vet. Med. 2011; 56, 319–324.

21. Balažová A., Blanáriková V., Bilka F., Bilková A., Kiňová Sepová, H. Efekt troch rôznych elicitorov na produkciu sanguinarínu suspenznými kultúrami nízko-morfínovej odrody maku siateho (Papaver somniferum L.). Čes. slov. Farm. 2011; 60, 237–240.

22. Bilková A., Kiňová Sepová H., Bilka F., Bukovský M., Balažová A., Bezáková, L. Identification of newly isolated lactobacilli from the stomach mucus of lamb. Acta Facult. Pharm. Univ. Comenianae 2008; 55, 64–72.

23. Kiňová Sepová H., Bilková A. Isolation and identification of new lactobacilli from goatling stomach and investigation of reuterin production in Lactobacillus reuteri strains. Folia Microbiol 2003; 58, 33–38.

24. Mlynarčík D., Bukovský M., Čupková V., Sirotková L. Praktické cvičenia z mikrobiológia a imunologických prípravkov. Skriptá, FaF UK Bratislava, 1995, 160.

25. Annuk H., Shchepetova J., Kullisaar, T., Songisepp E., Zilmer M., Mikelsaar M. Characterization of intestinal lactobacilli as putative probiotic candidates. J. Appl. Microbiol. 2003; 94, 403–412.

26. Hütt P., Shchepetova J., Lõivukene K., Kullisaar T., Mikelsaar M. Antagonistic activity of probiotic lactobacilli and bifidobacteria against entero- and uropathogens. J. Appl. Microbiol. 2006; 100, 1324–1332.

27. Vieira S. L., Oyarzabal O. A., Freitas D. M., Berres J., Pena J. E. M., Torres C. A. Performance of broilers fed diets supplemented with sanguinarine-like alkaloids and organic acid. J. Appl. Poult. Res. 2008; 17, 128–133.

28. Rawling M. D., Merrifield D. L., Davies S. J. Preliminary assessment of dietary supplementation of Sangrovit on red tilapia (Oreochromis niloticus) growth performance and health. Aquaculture 2009; 294, 118–122.

29. Jurkiewicz J., Gruzauskas R., Zdunczyk Z., Semaskaite A., Jankowski J., Totilas Z., Jarule V., Sasyte V., Zdunczyk P., Raceviciute-Stupeliene A., Svirmickas G. Effect of dietary addition of Macleaya cordata alkaloid extract on growth performance, caecal indices and breast meat fatty acids profile in male broilers. J. Anim. Physiol. Anim. Nutr. 2011; 95, 171–178.

30. Zdařilová A., Vrublová E., Vostalová J., Klejdus B., Stejskal D., Prosková J., Kosina P., Svobodová, A., Večera R., Hrbáč J., Černochová D., Vičar J., Ulrichová J., Šimánek V. Natural feed additive of Macleaya cordata: safety assessment in rats a 90-day feeding experiment. Food Chem Toxicol. 2008; 46, 3721–3726.

31. Nousiainen J., Javanainen P., Setala J., von Wright A. Lactic acid bacteria as animal probiotics. In: Salminen S., Ouwehand A., von Wright A. eds. Lactic acid bacteria: microbial and functional aspects 3rd ed. New York: Marcel Dekker 2004.

32. Caglar E., Sandalli N., Twetman S., Kavaloglu S., Ergeneli S., Selvi S. Effect of yogurt with Bifidobacterium DN-173 010 on salivary mutans streptococci and lactobacilli in young adults. Acta Odontol. Scand. 2005; 63, 317–320.

33. Caglar E., Cildir S. K., Ergeneli S., Sandalli N., Twetman S. Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws or tablets. Acta Odontol. Scand. 2006; 64, 314–318.

34. Tong H., Chen W., Merritt J., Qi F., Shi W., Dong X. Streptococcus oligofermentans inhibits Streptococcus mutans through conversion of lactic acid into inhibitory H2O2: a possible counteroffensive strategy for interspecies competition. Mol. Microbiol. 2007; 63, 872–880.

35. He X., Lux R., Kuramitsu H. K., Anderson M. H., Shi W. Achieving probiotic effects via modulating oral microbial ecology. Adv. Dent. Res. 2009; 21, 53–56.

36. Tanzer J., Livingston J., Thompson A. The microbiology of primary dental caries in human. J. Dent. Educ. 2001; 65, 1028–1037.

37. Švec P., Sedláček I., Žáčková L., Nováková D., Kukletová M. Lactobacillus spp. associated with early childhood caries. Folia Microbiol. 2009; 54, 53–58.

Labels
Pharmacy Clinical pharmacology
Topics Journals
Login
Forgotten password

Enter the email address that you registered with. We will send you instructions on how to set a new password.

Login

Don‘t have an account?  Create new account

#ADS_BOTTOM_SCRIPTS#