Human Hepatocyte II. – Cryopreservation

Czech version

Authors: Z. Dvořák; J. Adler ¹; J. Ulrichová
Authors‘ workplace: Ústav lékařské chemie a biochemie Lékařské fakulty Univerzity Palackého, Olomouc 1 Tkáňová banka Univerzitní nemocnice, Brno – Bohunice
Published in: Čes. slov. Farm., 2000; , 21-25
Category:

Overview

Human hepatocytes (HH) are an optimal in vitro model for the study of xenobiotics metabolism andtoxicity. However, there is still a problem of HH availability. A promising possibility for the storageof HH for later utilization is cryopreservation. Procedures and conditions used for cryopreservationof human hepatocytes and hepatocytes of other species are presented in the present paper. Comparedare some biochemical parameters characterizing viability and functionality of fresh and thawed HH.

Key words:
human hepatocyte – cryopreservation – viability – metabolism

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Article was published in

Czech and Slovak Pharmacy

2000 Issue 1