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Invertase in Immobilized Poppy Cells


Authors: J. Stano 1;  P. Kovács 2;  K. Neubert 3
Authors‘ workplace: Záhrada liečivých rastlín Farmaceutickej fakulty Univerzity Komenského, Bratislava 2 Katedra bunkovej a molekulárnej biológie liečiv Farmaceutickej fakulty Univerzity Komenského, Bratislava 3 Biochemický ústav Univerzity Martina Luthera, Halle/Saale 1
Published in: Čes. slov. Farm., 1999; , 30-33
Category:

Overview

Cell suspension culture of the opium poppy plant (Papaver somniferum L.) was permeabilized by Tween 80 and immobilized by glutaraldehyde. The pH optimum of the immobilized cells’ invertase is 4.6 and the temperature optimum is 50 °C. The enzyme hydrolysis was linear for 5 h, reaching 69 % of conversion. The immobilized cells are characterized by a high invertase activity and showed convenient mechanical properties.

Key words:
invertase – cell suspension culture – immobilization – permeabilization – opium poppy (Papaver somniferum L.)

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Pharmacy Clinical pharmacology
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