The Rabbit’s IOP Values after Instillation of the L-arginine.HCL with Selected Antiglaucomatic Drugs Mixtures Prepared in Vitro. (Comparative Study of Results of the Experimental Works)
Authors:
Z. Oláh; J. Veselovský
Authors‘ workplace:
Klinika oftalmológie LF UK v Bratislave, prednosta doc. MUDr. Vladimír Krásnik, Ph. D.
Published in:
Čes. a slov. Oftal., 68, 2012, No. 2, p. 77-81
Category:
Original Article
Overview
Purpose:
In this work the effect by interaction in vitro free amino acid L-arginine.HCl and selected antiglaucomatic drugs prepared mixtures on the physiological IOP values in rabbits is compare.
Methods:
The experimental works were performed on 5 female rabbits of the New Zealand White species. After instillation 2 drops of in vitro prepared 10% solution of the amino acid L-arginine.HCl with antiglacoma drugs (0.5% Timoptol, 0.005% Xalatan or 2% Trusopt) alone or in double combination mixtures at 8,00 am. into the left conjunctival sac the IOP was measured before and in 15th, 30th, 60th, 120th, 180th, 240th min. and 24 hours after instillation. The right eye was used as control.
Results:
The amino acid 10% L-arginine.HCl solution separately decrease the physiologic IOP in rabbit’s in confrontation with control eyes -2.9 torr. After antiglaucomatics separetly the decrease of the IOP were constated -0.69 torr by 0.5% Timoptol, -2.1 torr by 0.005% Xalatan, resp. -2.45 torr by 2% Trusopt. 10% L-arginine.HCl with combination of mono antiglaucomatics mixture decrease the physiologic IOP by 0.5% Timoptol -3.32 torr, by 2% Trusopt -4.45 torr, by 0,005% Xalatan only 2.91 torr. Application of 10% L-arginine-HCl in double combination of antiglaumatics decrease the physiologic IOP by 0.5% Timoptol & 0.005% Xalatan -2.96 torr, by 2% Trusopt & 0.005% Xalatan -4,32 torr and COSOPT (fixed combination of 0.5% Timolol maleat & 2% Dorsolamide.HCl) only -1.8 torr.
Conclusions:
We assume that the mentioned mixtures were able to decrease the physiologic IOP in rabbits through their better penetration to the receptors of the ciliary body. The mixture of the L-arginine.HCl with antiglaucomaticum Timoptol or Trusopt, resp. double combination of Timoptol & Xalatan or Trusopt & Xalatan was twice as effective as any of the mentioned antiglaucomatic drugs used separately. We conclude that the increased effectivity of the mixtures compared with the separate components is related to the instillation of the by now ready „bioantiglaucomatic”.
Key words:
Interaction in vitro of amino acid and antiglaucomatics. Effect of the amino acids and antiglaucomatic drug mixtures on the rabbit’s IOP
Sources
1. Bito, LZ., Daga, A., Blanco, J., Camras, CB.: Long-term maintenance of reduction intraocular pressure by daily or twice daily topical application of prostaglandins to cat or rhesus monkey eyes. Invest. Ophthalmol Vis Sci, 1983; 24: 312–319.
2. Choudhri, S., Wand, M., Shields, MB.: A comparison of dorzolamide-timolol combination versus the concomitant drugs. Am J Ophthalmol, 2000; 130: 832–833.
3. Chuman, H., Chuman, T., Nao-I, N., Sawada, A.: The effect of L-arginine on intraocular pressure in the human eye. Current Eye Res. 2000; 20: 511–516.
4. Harris, A., Jonescu-Cuypers, C.P., Kagemann,L., Nowacki, E.,A., Garzozi, H., Cole, C., Martin, B.: Effect of dorzolamide timolol combination versus timolol 0.5% on ocular blood-flow in patients with primary open-angle glaucoma. Am J Ophtalmol, 2001; 132: 490–495.
5. Kahán, IL.: Zur Biochemie des Auges. Akadémiai kiadó, Budapest, 1982, 113 s.
6. Kim CY., Whittington DA., Chang JS., Liao J., May JA., Christianson DW.: Structural aspects of isoenzyme selectivity in the binding of inhibitors to carbonic anhydrase II and IV. Med Chem, 2002; 45: 888–893.
7. Oláh, Z., Veselovský, J.: Vplyv aminokyseliny L-arginínu.HCl na aktivitu Trusoptu v komorovom moku. Transacta Ophthalmol Slovaca, 2003; 3, No.1: 5–8.
8. Oláh, Z., Veselovský, J., Gressnerová, S., Veselá, A.: The rabbitęs IOP after instillation of the amino acid L-arginine.HCl and antiglaucomatic mixture. Ophthalmic Res, 2004; 36, S1/04: 183.
9. Oláh, Z., Veselovský, J., Gressnerová, S., Veselá, A. (2005a): Vnútroočný tlak králikov po instilácii zmesi antiglaukomatika COSOPTu s aminokyselinou L-arginínom.HCl do spojovkového vaku. Čes a slov Oftal, 2005; 61: 3–12.
10. Oláh, Z., Veselovský, J., Gressnerová, S., Veselá, A.(2005b): Zmeny fyziologického VOT u králikov po aplikácii aminokyseliny lyzínu s antiglaukomatikom latanoprostom (Xalatanom). Čes a Slov Oftal, 2005; 61: 147–153.
11. Sugrue, M.F., Harris, A., Adamsons, I.: Dorzolamide hydrochloride: A topically active, carbonic anhydrase inhibitor for the treatment of glaucoma. Drugs of Today, 1997; 33: 283–298.
12. Sugrue, M.F.: Pharmacological and ocular hypotensive properties of topic carbonic anhydrase inhibitors. Prog. Retin. Eye Res, 2000; 19: 87–112.
13. Veselovský, J., Oláh, Z., Veselovská, Z., Veselá, A. (1998a): Mechanizmus účinku pilokarpínu v tkanivových štruktúrach predného segmentu oka z hľadiska jeho interakcie s voľnými aminokyselinami. Čs Oftal, 1998; 54: 10–17.
14. Veselovský, J., Oláh, Z., Veselovská, Z., Veselá, A.(1998b): Bioaktivita zmesi pilokarpínu s lyzínom na pupilu a vnútroočný tlak králikov. Čs Oftal, 1998; 54: 353–361.
15. Veselovský, J., Oláh, Z., Veselá, A., Gressnerová, S.: Fyziologický význam interakcie timololu s voľnými aminokyselinami v štruktúrach oka. Čes a Slov Oftal, 2002; 58: 143–148.
16. Veselovský, J., Oláh Z., Veselá, A., Gressnerova, S. (2003a): The free amino acids and the aqueous humor pH after antiglaucomatics in vitro. Bratisl Lek Listy, 2003; 104: 14–18.
17. Veselovský, J., Oláh, Z., Veselá, A., Gressnerová, S. (2003b): Zmes Timoptolu s L-arginínom.HCl: regulátor vnútroočného tlaku králikov. Čes a Slov Oftal, 2003; 59: 295–302.
18. Veselovský, J., Oláh, Z., Veselá, A., Gressnerová, S. (2004a): Vnútroočný tlak králikov po aplikácii 10% L-arginínu.HCl v 2% Trusopte. Čes a Slov Oftal, 2004; 60: 81–88.
19. Veselovský, J., Oláh, Z., Veselá, A., Gressnerová, S. (2004b): Reakcia fyziologického vnútroočného tlaku králika na Latanoprost (Xalatan) a jeho zmesi s aminokyselinou L-arginínom.HCl. Čes a Slov Oftal, 2004; 60: 319–327.
20. Veselovský, J., Oláh, Z., Veselá, A., Gressnerová, S.: Redukcia fyziologickej hodnoty VOT zmesou 2 aminokyselín (lyzínu a arginínu) v Timoptole v experimente u králika. Čes a Slov Oftal, 2006; 62: 3–10.
Labels
OphthalmologyArticle was published in
Czech and Slovak Ophthalmology
2012 Issue 2
Most read in this issue
- Repeatability and Reliability of the Visual Acuity Examination on logMAR ETDRS and Snellen Chart
- Acute Retinal Necrosis
- The Diabetic Macular Edema – New Possibilities of the Treatment
- Evaluation of Bacterial Colonization of the Conjunctival Sac in Ranibizumab Intravitreal Injections Treated Patients