Immunohistochemical Characterization of Collagen IV in Control Corneas and in Corneas Obtained from Patients Suffering from Posterior Polymorphous Corneal Dystrophy
Authors:
S. Merjavá 1; P. Lišková 1; K. Jirsová 1,2
Authors‘ workplace:
Laboratoř biologie a patologie oka, Institut dědičných metabolických chorob, VFN a 1. LF UK, Praha, vedoucí pracoviště prof. MUDr. Milan Elleder, DrSc.
1; Oční tkáňová banka, VFN a UK, Praha, vedoucí pracoviště MUDr. Marcela Černická, MBA
2
Published in:
Čes. a slov. Oftal., 64, 2008, No. 3, p. 115-119
Overview
The expression of all six chains of collagen IV was studied using the indirect fluorescent immunohistochemistry in seven control corneas and seven corneas obtained from patients suffering from the posterior polymorphous corneal dystrophy. Heterogeneous staining, especially in the epithelial basement membrane and Descemet’s membrane, was observed in the control corneas. An increase of the staining intensity for the α1 and α2 chains was observed, especially in the Descemet’s membrane and the corneal stroma in samples obtained from the patients compared to the control tissues.
Key words:
collagen IV, cornea, indirect fluorescent immunohistochemistry
Sources
1. Biswas, S., Munier, F.L., Yardley, J., et al: Missense mutations in COL8A2, the gene encoding the α2 chain of type VIII collagen, cause two forms of corneal endothelial dystrophy. Hum. Mol. Genetics, 21, 2001: 2415–2423.
2. Boruchoff, S.A., Kuwabara, T.: Electron microscopy of Posterior polymorphous degeneration. Am. J. Ophthalmol., 72, 1971: 879–887.
3. Cibis, G.W., Krachmer, J.H., Phelps, C.D., et al.: Iridocorneal adhesion in Posterior polymorphous corneal dystrophy. Trans. Am. Acad. Ophthalmol. Otolaryngol., 81, 1976: 770–777.
4. Cibis, G.W., Krachmer, J.H., Phelps, C.D., et al.: The clinical spectrum of Posterior polymorphous dystrophy. Arch. Ophthalmol., 95, 1977: 1529–1537.
5. Deng, Y., Liu, S., Cai, R.: Immunohistochemistry research on keratoconus. Yan Ke Xue Bao, 17, 2001: 65–67, 75.
6. Gwilliam. R., Liskova. P., Filipec. M., et al.: Micro heterogenity in Czech families with Posterior polymorphous corneal dystrophy mapping to chromosome 20p11.2 and the exclusion of the candidate gene VSX1. Invest. Ophthalmol. Vis. Sci., 46, 2005: 4480–4484.
7. Hogan, M.J., Bietti, G.: Hereditary deep dystrophy of the cornea (Polymorphous). Am. J. Ophthalmol., 68, 1969: 777–788.
8. Hudson, B.G., Reeders, S.T., Tryggvason, K.: Type IV collagen: structure, gene organization, and role in human diseases. Molecular basis of Goodpasture and Alport Syndromes and diffuse leiomyomatosis. J. Biol. Chem., 268, 1993: 26033–26036.
9. Jirsova, K., Merjava, S., Martincova, R., et al.: Immunohistochemical characterization of cytokeratins in the abnormal corneal endothelium of posterior polymorphous corneal dystrophy patients. Exp. Eye Res., 84, 2007: 680–686.
10. Kefalides, N.A.: Structure and biosynthesis of basement membranes. Int. Rev. Connect. Tissue Res., 6, 1973: 63–104.
11. Kenney, M.C., Nesburn, A.B., Burgeson, R.E., et al: Abnormalities of the extracellular matrix in keratoconus corneas. Cornea, 16, 1997: 345–351.
12. Krafchak, C.M., Pawar, H., Moroi, S.E., et al.: Mutations in TCF8 cause posterior polymorphous corneal dystrophy and ectopic expression of COL4A3 by corneal endothelial cells. Am. J. Hum. Genet., 77, 2005: 694–708.
13. Krachmer, J.H.: Posterior polymorphous corneal dystrophy: A disease characterized by epithelial-like endothelial cells which influence management and prognosis. Trans. Am. Ophthalmol. Soc., 83, 1985: 413–475.
14. Kurpakus, M.A., Stock, E.L., Jones, J.C.: The role of the basement membrane in differential expression of keratin proteins in epithelial cells. Dev. Biol., 150, 1992: 243–255.
15. Leinonen, A., Mariyama, M., Mochizuki, T., et al: Complete primary structure of the human type IV collagen alpha4(IV) chain. J. Biol. Chem., 269, 1994: 26172–26177.
16. Lemmink, H.H., Schroder, C.H., Monnens, L.A.H., et al.: The clinical spectrum of type IV collagen mutations. Hum. Mutat., 9, 1997: 477–499.
17. Levy, S.G., McCartney, A. C. E., Sawada, H., et al.: Descemet’s membrane in the Iridocorneal-endothelial syndrome: morphology and composition. Exp. Eye Res., 61, 1995: 323–333.
18. Liskova, P., Tuft, S.J., Gwilliam, R., et al.: Novel mutations in the ZEB1 gene identified in Czech and British patients with posterior polymorphous corneal dystrophy. Hum. Mutat., 28, 2007: 638.
19. Ljubimov, A.V., Burgeson, R.E., Butkowski, R.J., et al.: Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab. Invest., 72, 1995: 461–473.
20. Marshall, G.E., Konstas, A.G., Lee, W.R.: Immunogold fine structural localization of extracellular matrix components in aged human cornea. Graefe’s Arch. Clin. Exp. Ophthalmol., 229, 1991: 157–163.
21. Michelacci, Y.M.: Collagens and proteoglycans of the corneal extracellular matrix. Braz. J. Med. Biol. Res., 36, 2003: 1037–1046.
22. Nishida, T.: Cornea. In: Krachmer, J.H., Mannis, M.J., Holland, E.J. (eds.): Cornea (2nd edition). Elsevier MOSBY Inc., 2005, 3–26.
23. Ortega, N., Werb, Z.: New functional roles for non-collegenous domains of basement membrane collagens. J. Cell Sci., 22, 2002: 4201–4214.
24. Prockop, D.J., Kivirikko, K.I.: Collagens: molecular biology, diseases, and potentials for therapy. Annu. Rev. Biochem., 64, 1995: 403–434.
25. Rodrigues, M.M., Sun, T.T., Krachmer, J.H., et al.: Epithelization of the corneal endothelium in Posterior polymorphous dystrophy. Invest. Ophthalmol. Vis. Sci., 19, 1980: 832–835.
26. Tuori, A.J., Virtanen, I., Aine, E., et al.: The immunohistochemical composition of corneal basement membrane in keratoconus. Curr. Eye Res., 16, 1997: 792–801.
27. Waring, G.O.: Posterior collagenous layer of the cornea: ultrastructural classification of abnormal collagenous tissue posterior to Descemetęs membrane in 30 cases. Arch. Ophthalmol., 100, 1982: 122–134.
Labels
OphthalmologyArticle was published in
Czech and Slovak Ophthalmology
2008 Issue 3
Most read in this issue
- Bilateral Simultaneous Dacryocystorhinostomy and Conjunctivocystorhinostomy
- H-Test: a New Alternative of Bagolini Test for Younger Children
- Surgery of Duane Retraction Syndrome I: Comparison of Unilateral Recession and Resection versus Bilateral Medial Rectus Recessions
- The First Experience with Gold Eyelid Implants in Facial Nerve Palsies