Neoplastic Effect of Indomethacin in N-methyl-N-nitrosourea Induced Mammary Carcinogenesis in Female Rats
Authors:
P. Kubatka 1; K. Kalická 2; B. Bojková 2; I. Ahlers 2; E. Ahlersová 2; M. Péč 1
Authors‘ workplace:
Ústav lekárskej biológie, Jesseniova lekárska fakulta, Univerzita Komenského, Martin, Slovenská republika
1; Katedra fyziológie živočíchov, Ústav biologických a ekologických vied, Prírodovedecká fakulta, Univerzita P. J. Šafárika, Košice, Slovenská republika
2
Published in:
Klin Onkol 2012; 25(5): 359-363
Category:
Original Articles
Overview
Background:
High levels of prostaglandins found in many neoplastic tissues, especially in colon cancer and breast cancer, suggest a role of cyclooxygenase in the process of carcinogenesis.
Material and methods:
The aim of this study was to analyse the chemopreventive potential of non-steroidal inflammatory drug indomethacin and its combination with pineal hormone melatonin in rat mammary carcinogenesis induced by N-methyl-N-nitrosourea. Indomethacin was administered 3 times a week and melatonin 4 times a week, both substances in a concentration of 20 µg/ml of drinking water. Chemoprevention began approximately 2 weeks before carcinogen administration and lasted until the end of the experiment 25 weeks later.
Results:
Indomethacin administered alone and in combination with melatonin stimulated the growth of mammary tumors. We found a significant increase in the average tumor volume caused by indomethacin alone by 126%, and in combination with melatonin by 104% compared to the control group. Indomethacin administered alone increased the incidence of tumors by 21.5% (also in combination with melatonin) and reduced the tumor latency by 17 days compared to controls. Melatonin alone significantly reduced tumor volume in comparison with control animals. During the long-term administration, both substances were well tolerated by animals.
Conclusion:
Indomethacin, a predominant cyclooxygenase inhibitor-1, showed significant neoplastic effects in the prevention of N-methyl-N-nitrosourea induced rat mammary carcinogenesis. This finding is in strong contrast to our previous experiment, where indomethacin in 7,12-dimethylbenz(a)anthracene-induced rat mammary carcinogenesis revealed marked antineoplastic effects.
Key words:
rats – mammary carcinogenesis – chemoprevention – indomethacin – melatonin
Submitted:
23. 2. 2012
Accepted:
30. 4. 2012
Sources
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Labels
Paediatric clinical oncology Surgery Clinical oncologyArticle was published in
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