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External quality control of CD34+ cell assessment in Czech and Slovak Republic – long-term experience from eight years


Authors: D. Lysák 1;  M. Budina 2;  M. Holubová 1,3;  P. Jindra 1
Authors‘ workplace: Hematologicko-onkologické oddělení Fakultní nemocnice Plzeň 1;  SEKK, spol. s r. o., Pardubice 2;  Biomedicínské centrum LF Plzeň 3
Published in: Transfuze Hematol. dnes,25, 2019, No. 3, p. 258-263.
Category: Original Papers

Overview

Determination of CD34+ cells by flow cytometry is a basic method for assessing the quality of hematopoietic stem cell grafts and for planning their collection by leukapheresis. This method is based on multiparameter flow cytometry and is well-standardized internationally. The EQA program for CD34 + quantification has been available in the Czech and the Slovak Republic since 2010 with more than 20 laboratories involved. Evaluation of the 8-year experience has shown an excellent long-term success of participating laboratories ranging between 80–100 %, with sporadic fluctuations. Some of the participants who have been involved in the program from the beginning, achieve even 90% success rates. Reproducibility of measurement results is highly dependent on the amount of CD34+ cells in the sample. CV oscillates around 10 % if the CD34+ count is 10/μL or higher and increases by dozens of percent below this level. The program plays an important role in harmonizing CD34+ determination between transplant centres and increases the likelihood of obtaining accurate and comparable results.

Keywords:

variability – quality control – CD34+ – EQA


Sources

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