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Diagnosis of Cytomegalovirus Infection by the Polymerase ChainReaction and Antigenaemia in Patients after Allogenic Transplantation of Peripheral Stem Cells - Comparison of318 Paired Samples


Authors: J. Mayer;  D. Dvořáková;  M. Krahulová;  J. Vorlíček;  J. Adler 1
Authors‘ workplace: II. interní hematoonkologická klinika FN, Brno-Bohunice 1 Tkáňová banka FN, Brno-Bohunice
Published in: Čas. Lék. čes. 1999; : 436-440
Category:

Overview

Background.
Cytomegalovirus (CMV) represents a serious infectious complication in patients after allogenichaematopoietic stem cell transplantation. Timely initiation of the therapy at the time of incipient active CMV infection(preemptive therapy) is an important prevention of CMV disease. It is not clear, what is the best indicator of thisincipient CMV infection.Methods and Results. The significance of the detection of incipient CMV infection by CMV-antigenaemia andDNA-viraemia was compared in this study. DNA-viraemia was detected by the non-nested polymerase chain reaction(PCR). The sensitivity of nested and non-nested PCR was also compared experimentally. We tested 318 bloodsamples for antigenaemia and DNA-viraemia. Six samples were significantly positive for PCR, no sample wassignificantly positive for antigenaemia. Timely initiation of therapy resulted in the rapid eradication of the incipientactive CMV infection and clearance of DNA-viraemia.Conclusions. Non-nested PCR is a suitable and more reliable method for the monitoring of incipient active CMVinfection and it is a good method for timely initiation of preemptive antiviral therapy.

Key words:
CMV - cytomegalovirus, preemptive therapy, PCR - polymerase chain reaction, ganciclovir, foscarnet.

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Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management Dental Hygienist
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