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Properties of Staphylococcus aureus strains from food processing staff


Authors: T. Gelbíčová 1;  H. A. Tegegne 1,2;  M. Florianová 1;  I. Koláčková 1;  R. Karpíšková 1
Authors‘ workplace: Výzkumný ústav veterinárního lékařství, v. v. i., Hudcova 70, 621 00 Brno 1;  Veterinární a farmaceutická univerzita, Palackého 1946/1, 612 42 Brno 2
Published in: Epidemiol. Mikrobiol. Imunol. 67, 2018, č. 4, s. 161-165
Category: Original Papers

Overview

Aim:

The food processing staff may act as a reservoir of virulent strains of Staphylococcus aureus and contribute to their transmission to foods. The aim of this study was to assess the occurrence and properties of S. aureus in cheese factory staff.

Material and methods:

Throat and hand swabs collected from the staff of three different cheese factories were tested. The obtained isolates were characterized on the basis of detection of virulence factors, antimicrobial resistance testing, spa typing, and macrorestriction analysis.

Results:

S. aureus was detected in 58% of the hand swab samples (7/12) and in 47% (17/36) of the throat swab samples. No methicillin-resistant S. aureus was detected in the throat or on the hands of the food processing staff. Strains carrying genes responsible for the production of enterotoxins (58%) and/or toxic shock syndrome toxin (25%) were recovered from employees of all three premises. One throat swab isolate was positive for the gene encoding production of exfoliatin A. There was no clonal relationship between S. aureus strains isolated from the throat and hands, which suggests possible contamination of the employees' hands arising from the manufacturing environment.

Conclusion:

Good compliance with hygiene guidelines (washing and disinfecting hands and the environment regularly, using gloves and masks, etc.) is a necessary tool for reducing the risk of S. aureus spread by the employees working in the food industry.

Keywords:

staphylococcal enterotoxins– spa typing – macrorestriction analysis – virulence factors


Sources

1. Wertheim HF, Melles DC, Vos MC, et al. The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis, 2005;5(12):751–762.

2. Zecconi A, Scali F. Staphylococcus aureus virulence factors in evasion from innate immune defenses in human and animal diseases. Immunol Lett, 2013, 50(1-2):12–22. 

3. Wu S, Duan N, Gu H, et al. A review of the methods for detection of Staphylococcus aureus enterotoxins. Toxins, 2016;8(7). pii: E176. doi: 10.3390/toxins8070176.

4. Pinchuk IV, Beswick EJ, Reyes VE. Staphylococcal enterotoxins. Toxins, 2010;2(8):2177–2197.

5. Argudín MA, Mendoza MC, González-Hevia MA, et al. Genotypes exotoxin gene content, and antimicrobial resistance of Staphylococcus aureus strains recovered from foods and food handlers. Appl Environ Microbiol, 2012;78(8):2930–2935. 

6. Castro A, Santos C, Meireles H, et al. Food handlers as potential sources of dissemination of virulent strains of Staphylococcus aureus in the comunity. J Infect Public Health, 2016;9(2):153–160.

7. Ho J, Boost MV, O’Donoghue MM. Tracking sources of Staphylococcus aureus hand contamination in food handlers by spa typing. Am J Infect Control, 2015;43(7):759–761. 

8. den Heijer CD, van Bijnen EM, Paget WJ, et al. Prevalence and resistance of commensal Staphylococcus aureus, including methicillin-resistant S. aureus, in nine European countries: a cross-sectional study. Lancet Infect Dis, 2013;13(5):409–415. 

9. Baptistão LG, Silva NC, Bonsaglia EC, et al. Presence of immune evasion cluster and molecular typing of methicillin-susceptible Staphylococcus aureus isolated from food handlers. J Food Prot, 2016;79(4):682–686.

10. Aung MS, San T, Aye MM, et al. Prevalence and genetic characteristics of Staphylococcus aureus and Staphylococcus aureus isolates harboring Panton-Valentine leukocidin, enterotoxins, and TSST-1 genes from food handlers in Myanmar. Toxins, 2017;9(8). pii: E241. doi: 10.3390/toxins9080241.

11. Hallin M, Friedrich AW, Struelens MJ. spa typing for epidemiological surveillance of Staphylococcus aureus. Methods Mol Biol, 2009;551:189–202. 

12. Denayer S, Delbrassinne L, Nia Y, et al. Food-borne outbreak investigation and molecular typing: high diversity of Staphylococcus aureus strains and importance of toxin detection. Toxins, 2017;9(12). pii: E407. doi: 10.3390/toxins9120407.

13. Oliveira DC, De Lencastre H. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother, 2002;46(7):2155–2161.

14. Clinical and laboratory standards institute (2012): Performance standards for antimicrobial susceptibility testing. CLSI Document M100-S22. USA, Pa.

15. Monday SR, Bohach GA. Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates. J Clin Microbiol, 1999;37(10):3411–3414.

16. Løvseth A, Loncarevic S, Berdal KG. Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates. J Clin Microbiol, 2004;42(8):3869–3872.

17. Mehrota M., Wang G., Johnson WM. Multiplex PCR for detec-tion of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol, 2000;38(3):1032–1035.

18. Lina G, Piémont Y, Godail-Gamot F, et al. Involvement of Panton--Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis, 1999;29(5):1128–1132.

19. Hososaka Y, Hanaki H, Endo H, et al. Characterization of oxacillin-susceptible mecA-positive Staphylococcus aureus: a new type of MRSA. J Infect Chemother, 2007;13(2):79–86. 

20. Pantůček R, Götz F, Doškař J, et al. Genomic variability of Staphylococcus aureus and the other coagulase positive Staphylococcus species estimated by macrorestriction analysis using pulsed-field gel electrophoresis. Int J Syst Bacteriol, 1996; 46(1):216–222.

21. Shopsin B, Gomez M, Montgomery SO, et al. Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains. J Clin Microbiol, 1999;37(11):3556–3563.

22. Staphylococcus aureus MLST website sited at the University of Oxford (Jolley & Maiden 2010), BMC Bioinformatics., 11, s. 595. Dostupné na www:< https://pubmlst.org/ saureus/.

23. Jordá GB, Marucci RS, Guida AM, et al. Carriage and characterization of Staphylococcus aureus in food handlers. Rev Argent Microbiol, 2012;44(2):101–104.

24. Zeinhom MM, Abdel-Latef GK, Jordan K. The use of multiplex PCR to determine the prevalence of enterotoxigenic Staphylococcus aureus isolated from raw milk, feta cheese, and hand swabs. J Food Sci, 2015;80(12):M2932–2936.

25. Gelbíčová T, Tegegne HA, Tomáštíková Z, et al. Výskyt a šíření bakterií Staphylococcus aureus při výrobě extra tvrdého zrajícího sýra. Mlékařské listy, 2017;28(6):16–20.

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