IGRA methods in the routine operation – QuantiFERON^®-TB Gold or T-SPOT.TB?

Czech version

Authors: J. Amlerová; J. Hrabák
Authors‘ workplace: Ústav mikrobiologie a Biomedicínské centrum, Lékařská fakulta a Fakultní nemocnice v Plzni, Univerzita Karlova
Published in: Epidemiol. Mikrobiol. Imunol. 65, 2016, č. 4, s. 246-248
Category: Short Communication

Overview

For indirect diagnosis of tuberculosis, two commercial IGRA (Interferon Gamma Release Assay) assays are available – primal QuantiFERON^®-TB Gold test, new version QuantiFERON^®-TB Gold Plus test (four tube, differentiation in activity CD4+ a CD8+) and T-SPOT^®.TB test. Both methods are based on the same principle, but their workflows are different. In this article, both assays are compared on the collection of 284 patients. Inter-rate agreement measure showed 81.3% consistency and Cohen’s kappa index was calculated as 0.72. In case of discrepancy between IGRA and other methods (clinical aspects, X-ray diagnostic, etc.), results should be confirmed by second IGRA assay for correct interpretation.

KEYWORDS:
IGRA – Mycobacterium tuberculosis – Quantiferon – T-SPOT^®.TB

Sources

1. Andersen P, Munk ME, Pollock JM, et al. Specific immune-based diagnosis of tuberculosis. Lancet, 2000; 356(9235): 1099–1104.

2. Woo KS, Choi JL, Kim BR, et al. Significance of interferon-gamma response to mitogen in serial QuantiFERON-TB Gold In-Tube assay of routine laboratory practice. Clin Chim Acta, 2014; 430: 79–83.

3. NCCLS Approved Guideline. Performance of single Cell Immune Response Assays I/LA26-A [online], [cit. 2016-04-25]. Dostupné na www: <http://shop.clsi.org/site/Sample_pdf/ILA26A2_sample.pdf>

4. Tanguay S, Killion JJ. Direct comparison of ELISPOT and ELISA--based assays for detection of individual cytokine-secreting cells. Lymphokine Cytokine Res, 1994; 13(4): 259–263.

5. Favre N, Bordmann G, Rudin W. Comparison of cytokine measurements using ELISA, ELISPOT and semi-quantitative RT-PCR. J Immunol Methods, 1997; 204(1): 57–66.

6. Chee CBE, Gan SH, KhinMark W, et al. Comparison of Sensitivities of Two Commercial Gamma Interferon Release Assays for Pulmonary Tuberculosis. J Clin Microbiol, 2008; 46(6): 1935–1940.

7. Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics, 1977; 33(1): 159–174.

8. Connell TG, Ritz N, Paxton GA, et al. Comparison of Tuberculin Skin Testing, QuantiFERON-TB Gold and T-SPOT.TB in Children. PLoS ONE, 2008; 3(7): e2624.