The Use of IgG Antibody Avidity Assays in the Diagnosis of Cytomegalovirus Infection

Czech version

Authors: V. Štěpánová ¹; L. Plíšková ²; M. Kubišová ³; E. Štěpánová ⁴; R. Bolehovská ²; V. Boštíková ⁵; M. Svobodová ⁶
Authors‘ workplace: Ústav klinické mikrobiologie, NRL pro CMV, FN a LF UK Hradec Králové ¹; Ústav klinické biochemie a diagnostiky, FN a LF UK Hradec Králové ²; Gerontometabolická klinika, 2. interní klinika, FN a LF UK, Hradec Králové ³; 2. interní klinika FN a LF UK, Hradec Králové ⁴; Katedra epidemiologie a mikrobiologie Fakulty vojenského zdravotnictví Univerzity obrany, Hradec Králové ⁵; Oddělení klinické mikrobiologie Nemocnice Pardubice ⁶
Published in: Epidemiol. Mikrobiol. Imunol. 60, 2011, č. 3, s. 115-120

Overview

Human cytomegalovirus (CMV) is the most common cause of congenital infection. Primary CMV infection can lead to severe disease and complications in patients immunocompromised as a result of disease or therapy. IgG antibody avidity assays make it possible to differentiate between primary infection and reactivation of latent infection or reinfection. The study objective was to determine CMV IgG avidity by enzyme-linked immunosorbent assay (ELISA) with denaturation of IgG antibody binding to the antigen and by chemiluminiscent microparticle immunoassay (CMIA) on an Abbott Architect analyzer.

Both methods yielded comparable CMV IgG avidity results. In some cases, the Abbott test was superior in reflecting IgG antibody maturation during primary infection to microplate ELISA using antigen-antibody complex dissociation by a denaturing agent.

Key words:
cytomegalovirus (CMV) – IgG antibodies – avidity – primary infection

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Hygiene and epidemiology Medical virology Clinical microbiology

Amoebae of the Genus Acanthamoeba – Causative Agents of Human Infections

Vaccination against Viral Hepatitis A and B in Adults Aged over 40 Years – Antibody Persistence and Immune Memory

Prevention of Tick-Borne Meningoencephalitis – Summary Report

Article was published in

Epidemiology, Microbiology, Immunology

2011 Issue 3