#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

DRE-PCR (DoubleRepetitive Element-Polymerase Chain Reaction) – A New Molecular Epidemiologi-cal Method in Tuberculosis


Authors: Xet-Mull A.-M. 1;  M. Kubín 2,3;  V. Príkazský 2;  E. H. Harris 1;  L. W. Riley 1
Authors‘ workplace: School of Public Health, University of California in Berkeley, CA, USA 2 Státní zdravotní ústav, Praha 3 Hygienická stanice hlavního města Prahy
Published in: Epidemiol. Mikrobiol. Imunol. , 1999, č. 2, s. 82-86
Category:

Overview

In a pilot study Double Repetitive Element-Polymerase Chain Reaction 20 clinical is olates ofMycobacterium tuberculosis from Guatemala and 49 strains from Prague were typed. This techniqueis based on direct evidence of repetitive elements IS6110 or PGRS and does not require DNApurification, digestion by endonuclease nor Southern blot hybridization. Preliminary examinationof Guatemalian strains revealed a striking identity or similarity of DRE-PCR profiles while thePrague strains were characterized by conspicuous polymorphism. The Prague strains were exami-ned in a total number of 13 series of electrophoreograms and subsequently subjected to automatedanalysis with GelCompar software. The DRE-PCR method is suitable for screening of a majornumber of clinical isolates of M. tuberculosis in laboratories equipped with a minimum of technicalfacilities for the PCR method, furthermore it requires facilities for synthesis of the necessaryprimers and at least basic knowledge of molecular biology.

Key words:
DRE-PCR – Mycobacterium tuberculosis.

Full text is not available online.
If interested in a scan of this journal, contact NTO ČLS JEP.

Labels
Hygiene and epidemiology Medical virology Clinical microbiology

Article was published in

Epidemiology, Microbiology, Immunology


1999 Issue 2

Most read in this issue
Topics Journals
Login
Forgotten password

Enter the email address that you registered with. We will send you instructions on how to set a new password.

Login

Don‘t have an account?  Create new account

#ADS_BOTTOM_SCRIPTS#