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The possibilities of molecular testing of somatic aberrations in tumor tissue using NGS in routine practice - current situation in the Czech Republic


Authors: Pavel Dundr 1;  Radoslav Matěj 2,3;  Aleš Ryška 4;  Jana Prausová 5;  Jindřich Fínek 6;  Luboš Petruželka 7;  Ivana Stružinská 1
Authors‘ workplace: Ústav patologie 1. LF UK a VFN v Praze 1;  Ústav patologie, 3. LF UK a FN Královské Vinohrady 2;  Ústav patologie a molekulární medicíny, 3. LF UK a Fakultní Thomayerova nemocnice 3;  Fingerlandův ústav patologie, LF UK a FN Hradec Králové 4;  Onkologická klinika 2. LF UK a Fakultní nemocnice v Motole 5;  Onkologická a radioterapeutická klinika LF UK v Plzni a Fakultní nemocnice Lochotín 6;  Onkologická klinika 1. LF UK a VFN v Praze 7
Published in: Čes.-slov. Patol., 57, 2021, No. 3, p. 147-149
Category: Reviews Article

Overview

Molecular testing of tumor tissue for the detection of somatic aberrations using NGS is increasingly gaining significance in routine practice. The technical aspects of testing are standardized and currently do not pose a problem. However, the situation is evolving very rapidly regarding the indication of testing, which depends on the sometimes rapidly developing medical knowledge and needs in clinical practice. In order to implement NGS testing in practice and arrange its reimbursement by the health care system, first it is necessary to reach an agreement on the level of professional societies concerning the definition of priority and medically clearly justified areas in which molecular testing has a clear impact on therapeutical choices. The next step is to reach an agreement with the health insurance companies regarding NGS testing. The aim of this article is to provide an overview of the issue of routine tumor tissue testing using the NGS method covered by public health insurance, with a summary of the current situation in the Czech Republic. Only the testing of somatic aberrations in solid tumors performed at pathology departments is discussed. The issue of testing in haemato-oncological centres is not the subject of this review.

Keywords:

NGS – diagnostics – Prognosis – prediction


Sources

1. Matias-Guiu X, Stanta G, Carneiro F, et al. The leading role of pathology in assessing the somatic molecular alterations of cancer: Position paper of the european society of pathology. Virchows Arch 2020; 476(4): 491-497.

2. Mosele F, Remon J, Mateo J, et al. Recommendations for the use of next-generation sequencing (ngs) for patients with metastatic cancers: A report from the esmo precision medicine working group. Ann Oncol 2020; 31(11): 1491-1505.

3. Luchini C, Lawlor RT, Milella M, Scarpa A. Molecular tumor boards in clinical practice. Trends Cancer 2020; 6(9): 738-744.

4. Condorelli R, Mosele F, Verret B, et al. Genomic alterations in breast cancer: Level of evidence for actionability according to esmo scale for clinical actionability of molecular targets (escat). Ann Oncol 2019; 30(3): 365-373.

5. Li MM, Datto M, Duncavage EJ, et al. Standards and guidelines for the interpretation and reporting of sequence variants in cancer: A joint consensus recommendation of the association for molecular pathology, american society of clinical oncology, and college of american pathologists. J Mol Diagn 2017; 19(1): 4-23.

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Labels
Anatomical pathology Forensic medical examiner Toxicology

Article was published in

Czecho-Slovak Pathology

Issue 3

2021 Issue 3

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