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Assessment of Amphetamine and Metamphetamine in Human Hair by the


Authors: J. Lázničková;  M. Dědičová;  F. Vorel
Authors‘ workplace: Soudnělékařské oddělení, Nemocnice, České Budějovice
Published in: Čes.-slov. Patol., , 2000, No. 2, p. 26-29
Category:

Overview

The authors present a procedure of concurrent identification and quantification of amphetamineand metamphetamine in human hair. The method involves rinsing of the hair (distilled water 55°C, 0.1 M hydrochloric acid, distilled water to neutral reaction , methanol) drying in air, homoge-nization by cutting (1-2 mm long), alkaline hydrolysis (20 mg hair, 1 ml 1M sodium hydroxide, 55°C, 120 min.), neutralization with 1 M hydrochloric acid to pH=7, extracting benzoylation with2,3,4,5,6-pentafluorobenzoyl chloride (0.3 ml 1 M sodium hydroxide, 4 ml cyclohexane, 30 ul cyclo-hexylamine in cyclohexane of a concentration of 20 ng/ul - internal standard, 50 ul aqueoussolution of triethylamine hydrochloride concentration of 100 mg/ml - reaction catalyst and 10 ul ofderivation agent 2,3,4,5,6-pentafluorobenzoyl chloride dilution 1:10 , shaking for 5 mins. by handand leaving to stand for 10 mins. ), centrifugation (5 mins., 3000 rotations/min.), collection of 2 mlcyclohexane layer, its evaporation at 40 °C in nitrogen atmosphere and dilution with 100 ulcyclohexane.The derivated exctract was subjected to analysis by the GC-MS method. The proce-dure was used for segmentation analysis of hair of two subjects abusing metamphetamine forprolonged periods. The revealed concentrations varied within the range of 0.99-5.25 mg/kg meta-mphetamine and 0.13-0.73 mg/kg amphetamine.

Key words:
hair analysis - extractive pentafluorobenzoylation - gas chromatography -mass spec-

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Anatomical pathology Forensic medical examiner Toxicology
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