ImmunohistochemicalDetection of Cytokine Receptors on Cryostat Tissue Sections

Czech version

Authors: A. Kumberová; A. Jirásek ¹; K. Koubek ¹; M. Kolářová ²
Authors‘ workplace: Ústav hematologie a krevní transfuze, Praha, přednosta MUDr. RNDr. P. Jarolím, DrSc. 2 Hlavův 1. patologickoanatomický ústav 1. LF UK, Praha, přednosta prof. MUDr. P. Miřejovský, DrSc. ¹
Published in: Čes.-slov. Patol., , 1999, No. 1, p. 30-35
Category:

Overview

We have studied tissue expression of the cytokine receptors using a high sensitivitity biotin-strep-tavidin system on cryostat sections. We used a panel of monoclonal antibodies from the 6thInternational Workshop on Human Leukocyte Differentiation Antigens, namely CD25 (IL-2Ra),CD95 (FAS antigen), CD116 (GM CSFR), CD117 (SCFR), CD120a (TNFR I), CD120b (TNFR II),CD121a (IL-1R I), CDw123 (IL-3R), CD124 (IL-4R), CD126 (IL-6R), CD127 (IL-7R), CDw128 (IL-8R),CD130 (gpl130), CD131 (IL-3R), CD132 (IL-2Rg), CD134 (OC-40), CD135 (FLT3/FLK2). Examinedtissues (lymph nodes and spleens) were obtained from 12 patients with folicular non-Hodgkin´slymphoma, periferal T non-Hodgkin´s lymphoma, B lymphoma, myeloma, Hodgkin´s disease, twocases of T cell rich B-lymphoma, autoimmune haemolytic anemia and two cases of rudimentarytrombocytopenic purpura. Our results indicate that immunohistological technology using nativetissues on cryostat sections, monoclonal antibodies and the visualisation with biotin-streptavidinis a particularly suitable supplementary staining procedure for detection of the cytokine recep-tors in tissues.

Key words:
cytokine receptors - cryostat tissue sections - biotin-streptavidin

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Article was published in

Czecho-Slovak Pathology

1999 Issue 1