Extracellular miRNA – biogenesis, function and their use as biomarkers in rheumatic diseases.

1. Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 1993; 75(5): 843–54. PubMed PMID: 8252621.

2. Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, et al. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 2000; 403(6772): 901–6. doi: 10.1038/35002607. PubMed PMID: 10706289.

3. Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T. Identification of novel genes coding for small expressed RNAs. Science 2001; 294(5543): 853–8. doi: 10.1126/science.1064921. PubMed PMID: 11679670.

4. Filipowicz W, Bhattacharyya SN, Sonenberg N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nature reviews Genetics 2008; 9(2): 102–14. doi: 10.1038/nrg2290. PubMed PMID: 18197166.

5. Iorio MV, Croce CM. Causes and consequences of microRNA dysregulation. Cancer journal (Sudbury, Mass) 2012; 18(3): 215–22. Epub 2012/06/01. doi: 10.1097/PPO.0b013e318250c001. PubMed PMID: 22647357; PubMed Central PMCID: PMCPMC3528102.

6. Creemers EE, Tijsen AJ, Pinto YM. Circulating microRNAs novel biomarkers and extracellular communicators in cardiovascular disease? Circulation research 2012; 110(3): 483 95.

7. Krol J, Loedige I, Filipowicz W. The widespread regulation of microRNA biogenesis, function and decay. Nature reviews Genetics 2010; 11(9): 597–610. doi: 10.1038/nrg2843. PubMed PMID: 20661255.

8. Han J, Lee Y, Yeom KH, Nam JW, Heo I, Rhee JK, et al. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. Cell 2006; 125(5): 887–901. doi: 10.1016/j.cell.2006.03.043. PubMed PMID: 16751099.

9. Gregory RI, Yan KP, Amuthan G, Chendrimada T, Doratotaj B, Cooch N, et al. The Microprocessor complex mediates the genesis of microRNAs. Nature 2004; 432(7014): 235–40. doi: 10.1038/nature03120. PubMed PMID: 15531877.

10. Lund E, Guttinger S, Calado A, Dahlberg JE, Kutay U. Nuclear export of microRNA precursors. Science 2004; 303(5654): 95–8. doi: 10.1126/science.1090599. PubMed PMID: 14631048.

11. Kim VN. MicroRNA precursors in motion: exportin-5 mediates their nuclear export. Trends in cell biology 2004;14(4):156-9. PubMed PMID: 15134074.

12. Bhayani MK, Calin GA, Lai SY. Functional relevance of miRNA sequences in human disease. Mutation research 2012; 731(1–2): 14–9. Epub 2011/11/17. doi: 10.1016/j.mrfmmm.2011.10.014. PubMed PMID: 22085809.

13. Niederer F, Trenkmann M, Ospelt C, Karouzakis E, Neidhart M, Stanczyk J, et al. Down-regulation of microRNA-34a* in rheumatoid arthritis synovial fibroblasts promotes apoptosis resistance. Arthritis and rheumatism 2012; 64(6): 1771–9. doi: 10.1002/art.34334. PubMed PMID: 22161761.

14. Pratt AJ, MacRae IJ. The RNA-induced silencing complex: a versatile gene-silencing machine. The Journal of biological chemistry 2009; 284(27): 17897–901. doi: 10.1074/jbc.R900012200. PubMed PMID: 19342379; PubMed Central PMCID: PMC2709356.

15. Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 2005; 120(1): 15–20. doi: 10.1016/j.cell.2004.12.035. PubMed PMID: 15652477.

16. Eulalio A, Huntzinger E, Nishihara T, Rehwinkel J, Fauser M, Izaurralde E. Deadenylation is a widespread effect of miRNA regulation. Rna 2009; 15(1): 21–32. doi: 10.1261/rna.1399509. PubMed PMID: 19029310; PubMed Central PMCID: PMC2612776.

17. Esteller M. Non-coding RNAs in human disease. Nature reviews Genetics. 2011;12(12):861-74. doi: 10.1038/nrg3074. PubMed PMID: 22094949.

18. Calin GA, Dumitru CD, Shimizu M, Bichi R, Zupo S, Noch E, et al. Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukaemia. Proceedings of the National Academy of Sciences of the United States of America. 2002; 99(24): 15524-9. doi: 10.1073/pnas.242606799. PubMed PMID: 12434020; PubMed Central PMCID: PMCPMC137750.

19. Iorio MV, Piovan C, Croce CM. Interplay between microRNAs and the epigenetic machinery: an intricate network. Biochemical et Biophysica Acta (BBA)-Gene Regulatory Mechanisms 2010; 1799(10): 694–701.

20. Weber B, Stresemann C, Brueckner B, Lyko F. Methylation of human microRNA genes in normal and neoplastic cells. Cell cycle 2007; 6(9): 1001–5. Epub 2007/04/26. doi: 10.4161/cc.6.9.4209. PubMed PMID: 17457051.

21. Baylin SB, Jones PA. A decade of exploring the cancer epigenome - biological and translational implications. Nat Rev Cancer 2011; 11(10): 726–34. doi: 10.1038/nrc3130. PubMed PMID: 21941284; PubMed Central PMCID: PMCPMC3307543.

22. Tang R, Li L, Zhu D, Hou D, Cao T, Gu H, et al. Mouse miRNA-709 directly regulates miRNA-15a/16-1 biogenesis at the posttranscriptional level in the nucleus: evidence for a microRNA hierarchy system. Cell Res 2012; 22(3): 504–15. Epub 2011/08/25. doi: 10.1038/cr.2011.137. PubMed PMID: 21862971; PubMed Central PMCID: PMCPMC3292299.

23. Chim SS, Shing TK, Hung EC, Leung TY, Lau TK, Chiu RW, et al. Detection and characterization of placental microRNAs in maternal plasma. Clinical chemistry. 2008;54(3):482-90. doi: 10.1373/clinchem.2007.097972. PubMed PMID: 18218722.

24. Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K, et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell research. 2008; 18(10): 997–1006.

25. Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proceedings of the National Academy of Sciences of the United States of America. 2008; 105(30): 10513–8. doi: 10.1073/pnas.0804549105. PubMed PMID: 18663219; PubMed Central PMCID: PMCPMC2492472.

26. Michael A, Bajracharya SD, Yuen PS, Zhou H, Star RA, Illei GG, et al. Exosomes from human saliva as a source of microRNA biomarkers. Oral Dis 2010; 16(1): 34–8. doi: 10.1111/j.1601-0825.2009.01604.x. PubMed PMID: 19627513; PubMed Central PMCID: PMCPMC2844919.

27. Kosaka N, Izumi H, Sekine K, Ochiya T. microRNA as a new immune-regulatory agent in breast milk. Silence. 2010;1(1):7. doi: 10.1186/1758-907X-1-7. PubMed PMID: 20226005; PubMed Central PMCID: PMCPMC2847997.

28. Zubakov D, Boersma AW, Choi Y, van Kuijk PF, Wiemer EA, Kayser M. MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation. Int J Legal Med 2010; 124(3): 217–26. doi: 10.1007/s00414-009-0402-3. PubMed PMID: 20145944; PubMed Central PMCID: PMCPMC2855015.

29. Weber JA, Baxter DH, Zhang S, Huang DY, Huang KH, Lee MJ, et al. The microRNA spectrum in 12 body fluids. Clinical chemistry. 2010; 56(11): 1733–41. doi: 10.1373/clinchem.2010.147405. PubMed PMID: 20847327; PubMed Central PMCID: PMC4846276.

30. Kosaka N, Iguchi H, Ochiya T. Circulating microRNA in body fluid: a new potential biomarker for cancer diagnosis and prognosis. Cancer Sci. 2010; 101(10): 2087–92. doi: 10.1111/j.1349-7006.2010.01650.x. PubMed PMID: 20624164.

31. Cocucci E, Racchetti G, Meldolesi J. Shedding microvesicles: artefacts no more. Trends in cell biology. 2009;19(2):43-51. Epub 2009/01/16. doi: 10.1016/j.tcb.2008.11.003. PubMed PMID: 19144520.

32. Wang K, Zhang S, Weber J, Baxter D, Galas DJ. Export of microRNAs and microRNA-protective protein by mammalian cells. Nucleic acids research. 2010;38(20):7248-59. doi: 10.1093/nar/gkq601. PubMed PMID: 20615901; PubMed Central PMCID: PMCPMC2978372.

33. Arroyo JD, Chevillet JR, Kroh EM, Ruf IK, Pritchard CC, Gibson DF, et al. Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proceedings of the National Academy of Sciences. 2011; 108(12): 5003–8.

34. Turchinovich A, Weiz L, Langheinz A, Burwinkel B. Characterization of extracellular circulating microRNA. Nucleic acids research. 2011; 39(16): 7223–33. doi: 10.1093/nar/gkr254. PubMed PMID: 21609964; PubMed Central PMCID: PMCPMC3167594.

35. Vickers KC, Palmisano BT, Shoucri BM, Shamburek RD, Remaley AT. MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. Nature cell biology. 2011; 13(4) :423–33.

36. Wagner J, Riwanto M, Besler C, Knau A, Fichtlscherer S, Roxe T, et al. Characterization of levels and cellular transfer of circulating lipoprotein-bound microRNAs. Arterioscler Thromb Vasc Biol. 2013; 33(6): 1392–400. doi: 10.1161/ATVBAHA.112.300741. PubMed PMID: 23559634.

37. Maggi LB, Kuchenruether M, Dadey DY, Schwope RM, Grisendi S, Townsend RR, et al. Nucleophosmin serves as a rate-limiting nuclear export chaperone for the Mammalian ribosome. Molecular and cellular biology. 2008; 28(23): 7050-65.

38. Kosaka N, Iguchi H, Yoshioka Y, Takeshita F, Matsuki Y, Ochiya T. Secretory mechanisms and intercellular transfer of microRNAs in living cells. The Journal of biological chemistry. 2010;285(23):17442-52. doi: 10.1074/jbc.M110.107821. PubMed PMID: 20353945; PubMed Central PMCID: PMC2878508.

39. Kogure T, Lin WL, Yan IK, Braconi C, Patel T. Intercellular nanovesicle-mediated microRNA transfer: a mechanism of environmental modulation of hepatocellular cancer cell growth. Hepatology. 2011;54(4):1237-48. Epub 2011/07/02. doi: 10.1002/hep.24504. PubMed PMID: 21721029; PubMed Central PMCID: PMCPMC3310362.

40. Turchinovich A, Samatov TR, Tonevitsky AG, Burwinkel B. Circulating miRNAs: cell-cell communication function? Front Genet. 2013; 4: 119. doi: 10.3389/fgene.2013.00119. PubMed PMID: 23825476; PubMed Central PMCID: PMCPMC3695387.

41. Zernecke A, Bidzhekov K, Noels H, Shagdarsuren E, Gan L, Denecke B, et al. Delivery of microRNA-126 by apoptotic bodies induces CXCL12-dependent vascular protection. Sci Signal. 2009; 2(100): ra81. doi: 10.1126/scisignal.2000610. PubMed PMID: 19996457.

42. Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nature cell biology. 2007;9(6):654-9. doi: 10.1038/ncb1596. PubMed PMID: 17486113.

43. Mittelbrunn M, Gutierrez-Vazquez C, Villarroya-Beltri C, Gonzalez S, Sanchez-Cabo F, Gonzalez MA, et al. Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. Nat Commun. 2011; 2: 282. doi: 10.1038/ncomms1285. PubMed PMID: 21505438; PubMed Central PMCID: PMCPMC3104548.

44. Fabbri M, Paone A, Calore F, Galli R, Gaudio E, Santhanam R, et al. MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory response. Proceedings of the National Academy of Sciences of the United States of America 2012; 109(31): E2110-6. doi: 10.1073/pnas.1209414109. PubMed PMID: 22753494; PubMed Central PMCID: PMCPMC3412003.

45. Iguchi H, Kosaka N, Ochiya T. Secretory microRNAs as a versatile communication tool. Communicative & integrative biology. 2010; 3(5): 478–81. doi: 10.4161/cib.3.5.12693. PubMed PMID: 21057646; PubMed Central PMCID: PMC2974086.

46. Kota J, Chivukula RR, O’Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, et al. Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. Cell 2009; 137(6): 1005–17. doi: 10.1016/j.cell.2009.04.021. PubMed PMID: 19524505; PubMed Central PMCID: PMCPMC2722880.

47. Nagata Y, Nakasa T, Mochizuki Y, Ishikawa M, Miyaki S, Shibuya H, et al. Induction of apoptosis in the synovium of mice with autoantibody-mediated arthritis by the intraarticular injection of double-stranded MicroRNA-15a. Arthritis and rheumatism 2009; 60(9): 2677–83. doi: 10.1002/art.24762. PubMed PMID: 19714650.

48. Stanczyk J, Pedrioli DM, Brentano F, Sanchez-Pernaute O, Kolling C, Gay RE, et al. Altered expression of MicroRNA in synovial fibroblasts and synovial tissue in rheumatoid arthritis. Arthritis and rheumatism 2008; 58(4): 1001–9. doi: 10.1002/art.23386. PubMed PMID: 18383392.

49. Pauley KM, Satoh M, Chan AL, Bubb MR, Reeves WH, Chan EK. Upregulated miR-146a expression in peripheral blood mononuclear cells from rheumatoid arthritis patients. Arthritis research & therapy 2008; 10(4): R101. doi: 10.1186/ar2493. PubMed PMID: 18759964; PubMed Central PMCID: PMCPMC2575615.

50. Nakasa T, Shibuya H, Nagata Y, Niimoto T, Ochi M. The inhibitory effect of microRNA-146a expression on bone destruction in collagen-induced arthritis. Arthritis and rheumatism 2011; 63(6): 1582–90. doi: 10.1002/art.30321. PubMed PMID: 21425254.

51. Li YT, Chen SY, Wang CR, Liu MF, Lin CC, Jou IM, et al. Brief report: amelioration of collagen-induced arthritis in mice by lentivirus-mediated silencing of microRNA-223. Arthritis and rheumatism 2012; 64(10): 3240–5. doi: 10.1002/art.34550. PubMed PMID: 22674011.

52. Garchow BG, Bartulos Encinas O, Leung YT, Tsao PY, Eisenberg RA, Caricchio R, et al. Silencing of microRNA-21 in vivo ameliorates autoimmune splenomegaly in lupus mice. EMBO Mol Med 2011; 3(10): 605–15. doi: 10.1002/emmm.201100171. PubMed PMID: 21882343; PubMed Central PMCID: PMCPMC3258486.

53. Trajkovski M, Hausser J, Soutschek J, Bhat B, Akin A, Zavolan M, et al. MicroRNAs 103 and 107 regulate insulin sensitivity. Nature 2011; 474(7353): 649–53. doi: 10.1038/nature10112. PubMed PMID: 21654750.

54. Janssen HL, Reesink HW, Lawitz EJ, Zeuzem S, Rodriguez-Torres M, Patel K, et al. Treatment of HCV infection by targeting microRNA. The New England journal of medicine 2013; 368(18): 1685–94. doi: 10.1056/NEJMoa1209026. PubMed PMID: 23534542.

55. Ottosen S, Parsley TB, Yang L, Zeh K, van Doorn LJ, van der Veer E, et al. In vitro antiviral activity and preclinical and clinical resistance profile of miravirsen, a novel anti-hepatitis C virus therapeutic targeting the human factor miR-122. Antimicrobial agents and chemotherapy 2015; 59(1): 599–608. doi: 10.1128/AAC.04220-14. PubMed PMID: 25385103; PubMed Central PMCID: PMCPMC4291405.

56. Jopling CL, Yi M, Lancaster AM, Lemon SM, Sarnow P. Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA. Science 2005 ;309(5740): 1577–81. doi: 10.1126/science.1113329. PubMed PMID: 16141076.

57. Jopling CL, Schutz S, Sarnow P. Position-dependent function for a tandem microRNA miR-122-binding site located in the hepatitis C virus RNA genome. Cell Host Microbe 2008; 4(1): 77–85. doi: 10.1016/j.chom.2008.05.013. PubMed PMID: 18621012; PubMed Central PMCID: PMCPMC3519368.

58. Lanford RE, Hildebrandt-Eriksen ES, Petri A, Persson R, Lindow M, Munk ME, et al. Therapeutic silencing of microRNA-122 in primates with chronic hepatitis C virus infection. Science 2010; 327(5962): 198–201. doi: 10.1126/science.1178178. PubMed PMID: 19965718; PubMed Central PMCID: PMCPMC3436126.

59. Murata K, Yoshitomi H, Tanida S, Ishikawa M, Nishitani K, Ito H, et al. Plasma and synovial fluid microRNAs as potential biomarkers of rheumatoid arthritis and osteoarthritis. Arthritis research & therapy 2010; 12(3): 1.

60. Murata K, Furu M, Yoshitomi H, Ishikawa M, Shibuya H, Hashimoto M, et al. Comprehensive microRNA analysis identifies miR-24 and miR-125a-5p as plasma biomarkers for rheumatoid arthritis. PloS one. 2013; 8(7): e69118. doi: 10.1371/journal.pone.0069118. PubMed PMID: 23874885; PubMed Central PMCID: PMC3715465.

61. Filkova M, Aradi B, Senolt L, Ospelt C, Vettori S, Mann H, et al. Association of circulating miR-223 and miR-16 with disease activity in patients with early rheumatoid arthritis. Annals of the rheumatic diseases 2014;73(10):1898-904. Epub 2013/07/31. doi: 10.1136/annrheumdis-2012-202815. PubMed PMID: 23897768; PubMed Central PMCID: PMCPmc4173742.

62. Sode J, Krintel SB, Carlsen AL, Hetland ML, Johansen JS, Horslev-Petersen K, et al. Plasma MicroRNA Profiles in Patients with Early Rheumatoid Arthritis Responding to Adalimumab plus Methotrexate vs Methotrexate Alone: A Placebo-controlled Clinical Trial. The Journal of rheumatology 2018; 45(1): 53–61. doi: 10.3899/jrheum.170266. PubMed PMID: 29142030.

63. Hruskova V, Jandova R, Vernerova L, Mann H, Pecha O, Prajzlerova K, et al. MicroRNA-125b: association with disease activity and the treatment response of patients with early rheumatoid arthritis. Arthritis research & therapy 2016; 18(1): 124. doi: 10.1186/s13075-016-1023-0. PubMed PMID: 27255643; PubMed Central PMCID: PMCPMC4890522.

64. Duroux-Richard I, Pers Y-M, Fabre S, Ammari M, Baeten D, Cartron G, et al. Circulating miRNA-125b is a potential biomarker predicting response to rituximab in rheumatoid arthritis. Mediators of inflammation 2014; 2014.

65. Castro-Villegas C, Perez-Sanchez C, Escudero A, Filipescu I, Verdu M, Ruiz-Limon P, et al. Circulating miRNAs as potential biomarkers of therapy effectiveness in rheumatoid arthritis patients treated with anti-TNFalpha. Arthritis research & therapy 2015; 17: 49. Epub 2015/04/11. doi: 10.1186/s13075-015-0555-z. PubMed PMID: 25860297; PubMed Central PMCID: PMCPmc4377058.

66. Beyer C, Zampetaki A, Lin N-Y, Kleyer A, Perricone C, Iagnocco A, et al. Signature of circulating microRNAs in osteoarthritis. Annals of the rheumatic diseases 2014: annrheumdis-2013-204698.

67. Kong R, Gao J, Si Y, Zhao D. Combination of circulating miR-19b-3p, miR-122-5p and miR-486-5p expressions correlates with risk and disease severity of knee osteoarthritis. American journal of translational research 2017; 9(6): 2852–64. PubMed PMID: 28670374; PubMed Central PMCID: PMCPMC5489886.

68. Cuadra VMB, González-Huerta NC, Romero-Córdoba S, Hidalgo-Miranda A, Miranda-Duarte A. Altered expression of circulating microRNA in plasma of patients with primary osteoarthritis and in silico analysis of their pathways. PloS one 2014; 9(6): e97690.

69. Wang H, Peng W, Ouyang X, Li W, Dai Y. Circulating microRNAs as candidate biomarkers in patients with systemic lupus erythematosus. Translational research: the journal of laboratory and clinical medicine 2012;160(3):198-206. Epub 2012/06/12. doi: 10.1016/j.trsl.2012.04.002. PubMed PMID: 22683424.

70. Wang G, Tam L-S, Li EK-M, Kwan BC-H, Chow K-M, Luk CC-W, et al. Serum and Urinary Cell–free MiR-146a and MiR-155 in Patients with Systemic Lupus Erythematosus. The Journal of rheumatology 2010; 37(12): 2516-22.

71. Wang G, Tam L, Li E, Kwan B, Chow K, Luk C, et al. Serum and urinary free microRNA level in patients with systemic lupus erythematosus. Lupus 2011; 20(5): 493–500.

72. Carlsen AL, Schetter AJ, Nielsen CT, Lood C, Knudsen S, Voss A, et al. Circulating microRNA expression profiles associated with systemic lupus erythematosus. Arthritis & Rheumatism 2013; 65(5): 1324–34.

73. Navarro-Quiroz E, Pacheco-Lugo L, Navarro-Quiroz R, Lorenzi H, Espana-Puccini P, Diaz-Olmos Y, et al. Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PloS one. 2017; 12(11):e0187973. doi: 10.1371/journal.pone.0187973. PubMed PMID: 29136041; PubMed Central PMCID: PMCPMC5685598.

74. Sing T, Jinnin M, Yamane K, Honda N, Makino K, Kajihara I, et al. microRNA-92a expression in the sera and dermal fibroblasts increases in patients with scleroderma. Rheumatology 2012: kes120.

75. Koba S, Jinnin M, Inoue K, Nakayama W, Honda N, Makino K, et al. Expression analysis of multiple microRNAs in each patient with scleroderma. Experimental dermatology 2013; 22(7): 489–91.

76. Tanaka S, Suto A, Ikeda K, Sanayama Y, Nakagomi D, Iwamoto T, et al. Alteration of circulating miRNAs in SSc: miR-30b regulates the expression of PDGF receptor –. Rheumatology 2013;52(11):1963-72.

77. Chouri E, Servaas NH, Bekker CPJ, Affandi AJ, Cossu M, Hillen MR, et al. Serum microRNA screening and functional studies reveal miR-483-5p as a potential driver of fibrosis in systemic sclerosis. Journal of autoimmunity 2018. doi: 10.1016/j.jaut.2017.12.015. PubMed PMID: 29371048.

78. Huang C, Wei J, Chang W, Chiou S, Chou C, Lin Y, et al. Higher expression of whole blood microRNA-21 in patients with ankylosing spondylitis associated with programmed cell death 4 mRNA expression and collagen cross-linked C-telopeptide concentration. The Journal of rheumatology 2014; 41(6): 1104.

79. Prajzlerova K, Grobelna K, Husakova M, Forejtova S, Jungel A, Gay S, et al. Association between circulating miRNAs and spinal involvement in patients with axial spondyloarthritis. PloS one. 2017;12(9):e0185323. doi: 10.1371/journal.pone.0185323. PubMed PMID: 28938006; PubMed Central PMCID: PMCPMC5609864.

80. Perez-Sanchez C, Font-Ugalde P, Ruiz-Limon P, Lopez-Pedrera C, Castro-Villegas MC, Abalos-Aguilera MC, et al. Circulating microRNAs as potential biomarkers of disease activity and structural damage in ankylosing spondylitis patients. Human molecular genetics 2018; 27(5): 875–90. doi: 10.1093/hmg/ddy008. PubMed PMID: 29329380.

81. Misunova M, Salinas-Riester G, Luthin S, Pommerenke C, Husakova M, Zavada J, et al. Microarray analysis of circulating micro RNAs in the serum of patients with polymyositis and dermatomyositis reveals a distinct disease expression profile and is associated with disease activity. Clinical and experimental rheumatology 2016;34(1):17-24. PubMed PMID: 26574749.

82. Hirai T, Ikeda K, Tsushima H, Fujishiro M, Hayakawa K, Yoshida Y, et al. Circulating plasma microRNA profiling in patients with polymyositis/dermatomyositis before and after treatment: miRNA may be associated with polymyositis/dermatomyositis. Inflamm Regen 2018; 38: 1. doi: 10.1186/s41232-017-0058-1. PubMed PMID: 29321815; PubMed Central PMCID: PMCPMC5757292.