Hodnocení cytokinového imunobarvení u ovariálních novotvarů a endometriomů

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Authors: Rosekeila Simões Nomelini ^1,2; Priscylla Kelliny Aparecida Antão ¹; Millena Prata Jammal ¹; Cid Almeida De Lima ¹; Paula Carolina Arvelos Crispim ¹; Agrimaldo Martins-Filho ¹; Renata Margarida Etchebehere ³; Eddie Fernando Candido Murta ¹
Authors‘ workplace: Laboratory of Applied Sciences for Women (LaCam)/ Department of Gynecology and Obstetrics, Federal University of Triângulo Mineiro, Uberaba-MG, Brazil ¹; Graduate Program in Gynecology and Obstetrics of the Ribeirão Preto Medical School of the University of São Paulo (FMRP-USP), Ribeirão Preto, São Paulo, Brazil ²; Surgical Pathology Service, Federal University of Triângulo Mineiro, Uberaba-MG, Brazil ³
Published in: Ceska Gynekol 2024; 89(5): 377-384
Category: Original Article
doi: https://doi.org/10.48095/cccg2024377

Overview

Cíl: Cílem naší studie bylo kvantifikovat a porovnat imunobarvení IL-2, IL-5, IL-6, IL-8 a TNFa ve tkáni endometriomu, nenádorových nádorech, benigních novotvarech a maligních novotvarech vaječníků. Materiál a metody: Studie se zúčastnilo 90 pacientek; bylo dia gnostikováno 15 nenádorových lézí ovaria, 28 ovariálních benigních novotvarů, 28 ovariálních maligních novotvarů a 19 ovariálních endometriomů. Imunohistochemie byla provedena pro cytokiny IL-2, IL-5, IL-6, IL-8 a TNFa a jejich koncentrace byly v těchto skupinách porovnány. Použili jsme Fisherův exaktní test, podle něhož jsou významné hodnoty p < 0,05. Výsledky: Imunobarvení epitelu na přítomnost IL-5 a IL-8 je silnější u endometriomů než u karcinomu vaječníků (p hodnoty 0,0046 a 0,0149). Stromální imunobarvení TNFa, IL-5, IL-6 a IL8 je silnější u endometriomů než u rakoviny vaječníků (p hodnoty 0,0008; < 0,0001; 0,0003 a 0,0006). Závěr: Silnější imunobarvení ně kte rých cytokinů u endometriomů ve srovnání s karcinomem ovaria odráží zánětlivou a imunitní odpověď, která by mohla být budoucím cílem nových objevů o infiltrativním chování endometriózy.

Klíčová slova:

cytokiny – Endometrióza – imunologie – ovariální novotvary

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ORCID authors

R. Simões Nomelin 0000-0001-6591-7619

P. K. Aparecia Antão 0000-0002-4294-5892

M. Prata Jammal 0000-0003-2693-8817

C. Almeida de Lima 0000-0002-6926-558X

P. C. Arvelos Crispim 0000-0003-2637-2462

A. Martins-Filho 0000-0002-2519-5583

R. M. Etchebehere 0000-0002-2241-5966

E. F. Candido Murta 0000-0003-4014-1345

Submitted/Doručeno: 28. 4. 2024

Accepted/Přijato: 30. 4. 2024

Prof. Rosekeila Simões Nomelini, MD