Examination of the Activity of Some Aminopeptidases in Immobilized Cells of the PoppyPlant
Authors:
J. Stano 1; K. Neubert 2; L. Hegedüs 3; F. Andriamainty 4
Authors‘ workplace:
Záhrada liečivých rastlín Farmaceutickej fakulty Univerzity Komenského, Bratislava 2 Biochemický ústav Univerzity Martina Luthera, Halle/Saale 3 EBA, Bratislava 4 Katedra farmaceutickej chémie Farmaceutickej fakulty Univerzity Komenského, Bratislava
Published in:
Čes. slov. Farm., 1999; , 136-139
Category:
Overview
Suspension cultures of the poppy plant (Papaver somniferum L.) were permeated with Tween 80and immobilized with glutaraldehyde. The values of optimum pH of L-alanine aminopeptidase(L-Ala-AP), L-tyrosine aminopeptidase (L-Tyr-AP) and L-arginine aminopeptidase (L-Arg-AP) innative and immobilized cells are 8.0; 7.0 and 7.8, respectively. In the cells immobilized withglutaraldehyde, the activity of the enzymes under study is very low in comparison with native cells.In the immobilization of amino peptidases, alginate gel was successfully applied.
Key words:
amino peptidases – suspension cell cultures – immobilization with glutaraldehydeand alginate – Papaver somniferum
Labels
Pharmacy Clinical pharmacologyArticle was published in
Czech and Slovak Pharmacy
1999 Issue 3
Most read in this issue
- Several Common Gastrointestinal Symptoms and their Effective and Safe Treatment
- Chiral Compounds and Their Pharmacological Effects
- Pharmaceutical Significance of the Species of the Genus Solidago2. The Saponins of the Species Solidago canadensis L. and Solidago gigantea Ait. var.serotina (O. Kuntze) Cronquist
- Investigation of the Effects of Ecological Aspects on the Content of Toxic Metals – Pb andCd – in the Drugs Flos tiliae and Flos sambuci