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A New Method of Detection of Plant Extracellular Proteolytic Enzymes


Authors: J. Stano 1;  I. Šafařík 2;  P. Kovács 3;  K. Neubert 4;  M. Šafaříková 2
Authors‘ workplace: Záhrada liečivých rastlín Farmaceutickej fakulty Univerzity Komenského, Bratislava 2 Laboratoř biochemie a biotechnologie Ústavu ekologie krajiny České akademie věd, České Budějovice 3 Katedra bunkovej a molekulárnej biológie liečiv Farmaceutickej fakulty 1
Published in: Čes. slov. Farm., 1998; , 271-272
Category:

Overview

By means of an insoluble chromogenic substrate (black gelasphere), a simple, sensitive and repro- ducible method of detection of extracellular proteolytic enzymes was worked out. Extracellular proteolytic enzymes (produced by a callus or suspension culture) hydrolyse black gelasphere. Colouring around the callus culture and under it is a sign of the activity of extracellular proteolytic enzymes produced by the callus culture. The intensity of colouring of the cultivating medium is a measure of the activity of extracellular proteolytic enzymes of the suspension culture.

Key words:
extracellular proteolytic enzymes – insoluble chromogenic substrate – black gelasphere – Chelidonium majus L.

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