#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

The Use of Molecular Genetics Techniques in Clinical Microbiology – Final Report from the Workshop of the Molecular Microbiology Working Group TIDE


Authors: J. Hrabák 1;  M. Bunček 2;  M. Dendis 3;  R. Horváth 3,9;  A. Chroňáková 4;  A. Libra 2;  J. Nešvera 5;  R. Pantůček 6;  N. Piskunová 7;  L. Plíšková 8;  F. Růžička 9;  P. Sauer 10;  I. Sedláček 6;  P. Trubač 7;  E. Žampachová 7;  H. Žemličková 11;  J. Scharfen 12
Authors‘ workplace: Ústav mikrobiologie, Lékařská fakulta UK a Fakultní nemocnice v Plzni, Plzeň 1;  Generi Biotech, Hradec Králové 2;  GeneProof, a. s., Brno 3;  Ústav půdní biologie, Biologické centrum ČAV, České Budějovice 4;  Mikrobiologický ústav ČAV, Praha 5;  Ústav experimentální biologie, Přírodovědecká fakulta, Masarykova univerzita, Brno 6;  Nemocnice České Budějovice, České Budějovice 7;  Lékařská fakulta UK a Fakultní nemocnice v Hradci Králové, Hradec Králové 8;  Mikrobiologický ústav FN u sv. Anny a LF MU, Brno 9;  Ústav mikrobiologie, Lékařská fakulta UP a Fakultní nemocnice v Olomouci, Olomouc 10;  Národní referenční laboratoř pro antibiotika, Státní zdravotní ústav v Praze, Praha 11;  Národní referenční laboratoř pro patogenní aktinomycety, Nemocnice Trutnov, Trutnov 12
Published in: Epidemiol. Mikrobiol. Imunol. 59, 2010, č. 3, s. 103-106

Overview

In the last decade, there has been a rapid development in the use of molecular genetics methods in clinical microbiology. Novel technologies bring new knowledge and approaches to various disciplines of microbiology – taxonomy, identification of microbes, clinical diagnosis, epidemiology of infectious diseases and antibiotic resistance. This article summarizes the conclusions from the workshop of the Molecular Microbiology Working Group TIDE held during the Second Annual Meeting of the Society for Medical Microbiology of the J. E. Purkyne Czech Medical Association.

Key words:
molecular microbiology – typing – identification – taxonomy – PCR – clinical microbiology.


Sources

1. Vandamme, P., Pot, B., Gillis, M., De Vos, P. et al. Polyphasic taxonomy, a konsensus approach to bacterial systematics. Microbiol. Rev., 1996, 60, p. 407–438.

2. Van Belkum, A., Tassios, P. T., Dijkshoorn, L., Haeggman, S. et al. Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin. Microbiol. Infect., 2007, 13, Suppl. 3, p. 1–46.

3. Čekanová, L., Kolář, M., Chromá, M., Sauer, P. et al. Prevalence of ESBL-positive bacteria in the community in the Czech Republic. Medical Science Monitor, 2009, 15, p. 202–206.

4. Grundmann, H., Aanensen, D. M., van den Vijngaard, C. C., Spratt, B. G. et al. Geographic distribution of Staphylococcus aureus causing invasive infections in Europe: a molecular-epidemiological analysis. PloS Med., 2010, 7, 1, p. e100215.

5. Hrabák, J., Empel, J., Gniadkowski, M., Halbhuber, Z. et al. CTX-M-15-producing Shigella sonnei from a Czech patient who traveled in Asia. Journal of Clinical Microbiology, 2008, 46, p. 2147–2148.

6. Hrabák, J., Empel, J., Bergerová, T., Fajfrlík, K. et al. International clones of Klebsiella pneumoniae and Escherichia coli with extended-spectrum -lactamases (ESBLs) in a Czech hospital. Journal of Clinical Microbiology, 2009, 47, p. 3353–3357.

7. Kolář, M., Sauer, P., Faber, E., Kohoutová, J. et al. Prevalence and spread of Pseudomonas aeruginosa and Klebsiella pneumoniae strains in patients with hematological malignancies. New Microbiologica, 2009, 32, p. 67–76.

8. Mariapia, V. M. (Ed.). Detection of Bacteria, Viruses, Parasites and Fungi. Springer, 2010.

9. Zemlickova, H., Urbaskova, P., Jakubu, V., Motlova, J. et al. Clonal distribution of invasive pneumococci, Czech Republic 1996–2003. Emerg. Infect. Dis., 2010, 16, p. 287–289.

Labels
Hygiene and epidemiology Medical virology Clinical microbiology

Article was published in

Epidemiology, Microbiology, Immunology

Issue 3

2010 Issue 3

Most read in this issue
Topics Journals
Login
Forgotten password

Enter the email address that you registered with. We will send you instructions on how to set a new password.

Login

Don‘t have an account?  Create new account

#ADS_BOTTOM_SCRIPTS#